Unformatted text preview: to convert diffracted light passing through the specimen to light vs dark contrast. e. Describe in general terms how the techniques of scanning confocal and deconvolution fluorescence microscopy can improve image resolution over that of conventional fluorescence microscopy. ( No need to compare the details of the two techniques, they both accomplish the same thing) (2 pts) Both of these technical modifications of std fluor. microscopy either physically (confocal) or mathematically (deconvolution) subtract out the fluorescence emissions that come from the fluorescence that is out of the focal plane. Therefore, the observer-detector sees only the fluor. that is in focus = sharper image. Page 1 Name ________________________ 1. For each of the items listed below, describe: (8 pts) Demo...
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- Spring '08
- oil immersion lens, differential interference contrast, microscope light source, deconvolution fluorescence microscopy