bis_104_mid_i_exam_key_b08 - Page 2

bis_104_mid_i_exam_key_b08 - Page 2 - b Degree of...

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5. What information does the technique of freeze-fracture replication provide regarding bio-membrane (e.g., plasma membrane) composition? (2) Reveals the presence or absence of integral (transmembrane) proteins; the abundance, relative size, and their distribution pattern within the inner and outer leaflets of the membrane . 6. What microscopic technique is used to view membrane specimens prepared by freeze-fracture replication? (2) Electron microscopy. (specifically, scanning) (SEM) 7. What two structural features of membrane lipids influence the relative fluidity of a bio-membrane? (2) a ) phospholipid fatty acid chain length: longer chains = less fluidity (greater viscosity
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Unformatted text preview: ) b ) Degree of saturation of phospholipid fatty acid chains: less saturation (more double bonds) = greater fluidity. ( amount of cholesterol in the membrane was also accepted, although this does not answer this question about the structure of membrane lipids) 8. What influence does cholesterol have on the transition temperature of a bio-membrane? (2) Cholesterol acts as a “buffer” to broaden the transition range so a membrane can remain functional over a broader range of temps. (In general, increased cholesterol decreases membrane permeability and fluidity). Page 1 Name ______________________________ Demo...
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