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bis_104_mid_i_exam_key_b08 - Page 4

bis_104_mid_i_exam_key_b08 - Page 4 - scanning confocal...

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9. Mukouyama used scanning confocal fluorescence microscopy to view fluorescently labeled markers in mouse limb skin samples. What advantage does this technique have over conventional fluorescence microscopy? (4) The scanning confocal system removes all of the unfocussed fluorescence from above and below the focal plane. The result is a much sharper, clear image of the specimen at each focal plane. 10. Embryonic (E15.5) limb skin samples from both normal and Ngn1 -/- : Ngn2 -/- double homozygous mutant mice were stained with fluorochrome- labeled antibodies for peripheral sensory nerves (2H3), undifferentiated endothelial cells (PECAM-1) and arterial endothelial cells (CX40). Stained samples were examined by tri-color
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Unformatted text preview: scanning confocal fluorescence microscopy. (10) Describe the RESULTS that were obtained. Normal: Staining of sensory nerves (2H3) and arterial (CX40) staining patterns were co-incident with each other. PECAM-1 staining showed normal background network of relatively undifferentiated endothelial cells. Ngn-1/2 mutant : Lacked significant nerve (2H3) staining and CX40 staining was absent or greatly reduced. PECAM-1 staining was normal as in wild type mice. What may be CONCLUDED from these results? (be sure to use precise Rules of Evidence terminology). Peripheral sensory nerves are required (necessary) for arterial endothelial cell differentiation (branching and CX40 expression) . Demo...
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