This preview shows page 1. Sign up to view the full content.
Unformatted text preview: by light microscopy. c. compare proliferation rate of transfected cells (those expressing B-gal) to that of normal cells (or tumor cells). RESULTS: (4) Cells expressing B-gal (erbB2) divide more frequently than normal cells (the same cell population prior to transfection). Describe one essential positive control for your experiment and provide the expected results . (10) CONTROL: (6) Verify that B-gal positive transfected cells actually co-express more erbB2 by measuring erbB2 protein on the PM directly: treat cells with a labeled antibody specific for erbB2 and quantify label or extract all PM proteins and quantify (e.g., western blot). RESULTS OF CONTROL: (4) Proliferating cells that are B-gal positive do in fact have elevated levels of erbB2 protein in their PMs. Demo...
View Full Document
This note was uploaded on 05/24/2010 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.
- Spring '08