Unformatted text preview: cells. OR: Biochemical approach: a. Start with pure populations of normal and cancer cells. b. Isolate and purify PMs from both ell types. c. do a total protein extraction from the membrane preps. d. separate extracted proteins by SDS-PAGE; do western blot and probe for erbB2 with labeled antibody. e. compare density of labeled protein bands on blot. Describe the results you would expect to obtain if your hypothesis were proven to be correct. (4) Cells taken from tumor tissue would show more intense fluorescence when compared to cells from normal tissue. OR: PM protein extracts from cancer cells would yield higher concentration of erbB2 protein per cell as revealed by western blot of normal vs cancer cell PMs. Demo...
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- Spring '08
- molecular biology, cancer cells