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Unformatted text preview: Apply a fluorescently labeled antibody for arterial cell markers such as EphrinB2 or CX40. Wash away any unbound antibody and examine cells by fluorescence microscopy . RESULTS OF EXPERIMENT If differentiation has occurred, endothelial cells should show a positive reaction (fluorescence) with the anti-CX-40 and anti-EphrinB2 antibodies . CONTROL: Purify and culture PECAM-1 positive endothelial cells alone, without Schwann cells. Treat with labeled antibodies as in experiment and observe. RESULTS OF CONTROL No reaction (no fluorescense) with anti-arterial marker antibodies. Demo...
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- Spring '08