bis_104_sample_exam_key_07 - Page 4

bis_104_sample_exam_key_07 - Page 4 - NAME _ PAGE 4 Now you...

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NAME ______________________________ PAGE 4 Now you would like to know more about the properties of the endothelial cell receptor protein that binds and responds to VEGF. You know from previous biochemical studies that the VEGF receptor has a molecular weight of 80,000 daltons.(80 Kd) and that it is the only protein in the membrane of these cells with that molecular weight. You hypothesize that this receptor is an integral (transmembrane) protein that has an extracellular domain. Design an experiment using SDS-PAGE as the assay to test this hypothesis. Outline the series of steps in your experimental procedure and sketch and label the resulting SDS-PAGE
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