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Unformatted text preview: 4. Probe the blots with an enzyme-linked antibody that is specific for the CTFR protein; develop the blot and compare staining pattern of PM proteins from normal vs CF cells. RESULTS: Plasma membrane extract from normal cells will give a single positive band corresponding to a mol wt of ~ 210kd. There will be no band in the transblot from CF cells. e. What additional information about the CFTR protein in normal cells could you obtain by making f reeze-fracture replicas of the cells and examining these with electron microscopy? This procedure would reveal which side of the plasma membrane (inner vs outer leaflet) the CFTR protein was embedded in (bumps vs dimples). It would also allow one to see whether the protein was arranged in clusters, or distributed diffusely around the cell, or if it was localized to one region of the cell. Demo...
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- Spring '08