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1 Lecture 14 Outline of lecture 14 Types of RNA Central dogma of genetics, flow of genetic information in the cell Peptide bond Colinearity of DNA, RNA and polypeptide Comparison of the prokaryotic and eukaryotic transcription and translation RNA polymerases Prokaryotic promoter Prokaryotic transcription : initiation elongation termination Eukaryotic transcription: initiation elongation termination Transcript cotranscriptional processing Intron splicing Small interfering RNAs A-PDF Split DEMO : Purchase from www.A-PDF.com to remove the watermark
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5 TRANSCRIPTION RNA polymerase Prokaryotes : only one Eukaryotes : Pol I : 18S-5.8S-26S rRNA genes Pol II : most of the genes Pol III : small RNA genes (e.g., 5S, tRNA) PHASES OF TRANSCRIPTION : INITIATION: It is mediated by the promoter of a gene. for RNA Pol I and II the promoter precedes a gene for RNA Pol III the promoter is a part of a gene Prokaryotic Promoters RNA polymerase binding sites -35 and -10, +1 start of transcription E. coli consensus PROMOTER sequences:
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This note was uploaded on 05/24/2010 for the course BIS BIS102 taught by Professor Hilt during the Winter '08 term at UC Davis.

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