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Unformatted text preview: PLEASE SCROLL DOWN FOR ARTICLE This article was downloaded by: [Universiteit Gent] On: 25 February 2010 Access details: Access Details: [subscription number 915540801] Publisher Informa Healthcare Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37- 41 Mortimer Street, London W1T 3JH, UK Biotechnic and Histochemistry Publication details, including instructions for authors and subscription information: http://www.informaworld.com/smpp/title~content=t713692932 Sihler's whole mount nerve staining technique: a review L. Mu a ; I. Sanders b a Upper Airway Research Laboratory, Department of Research, Hackensack University Medical Center, Hackensack, NJ b Alice and David Jurist Institute for Biomedical Research, Hackensack University Medical Center, First published on: 01 July 2009 To cite this Article Mu, L. and Sanders, I.(2009) 'Sihler's whole mount nerve staining technique: a review', Biotechnic and Histochemistry,, First published on: 01 July 2009 (iFirst) To link to this Article: DOI: 10.1080/10520290903048384 URL: http://dx.doi.org/10.1080/10520290903048384 Full terms and conditions of use: http://www.informaworld.com/terms-and-conditions-of-access.pdf This article may be used for research, teaching and private study purposes. Any substantial or systematic reproduction, re-distribution, re-selling, loan or sub-licensing, systematic supply or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material. Sihler’s whole mount nerve staining technique: a review L Mu 1 , I Sanders 2 1 Upper Airway Research Laboratory, Department of Research, Hackensack University Medical Center, Hackensack, NJ 07601 , 2 Alice and David Jurist Institute for Biomedical Research, Hackensack University Medical Center, New Jersey (07047) Abstract Sihler’s stain is a whole mount ner v e staining technique that renders other soft tissue translucent or transparent while staining the ner v es. It permits mapping of entire ner v e supply patterns of organs, skeletal muscles, mucosa, skin, and other structures after the specimens are fixed in neutralized formalin, macerated in potassium hydroxide, decalcified in acetic acid, stained in Ehrlich’s hematoxylin, destained in acetic acid, and cleared in glycerin. The unique ad v antage of Sihler’s stain o v er other anatomical methods is that all the ner v es within the stained specimen can be v isualized in their three-dimensional positions. To date, Sihler’s stain is the best tool for demonstrating the precise intramuscular branching and distribution patterns of skeletal muscles,...
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This note was uploaded on 05/28/2010 for the course BIOLOGY 03234 taught by Professor Sochacka during the Spring '10 term at Ghent University.
- Spring '10