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Unformatted text preview: Video Article Dissection of 6.5 dpc Mouse Embryos Kelly Shea, Niels Geijsen Massachusetts General Hospital, Harvard Stem Cell Institute, Harvard Medical School URL: http://www.jove.com/index/Details.stp?ID=160 DOI: 10.3791/160 Citation: Shea K., Geijsen N. (2007). Dissection of 6.5 dpc Mouse Embryos. JoVE. 2. http://www.jove.com/index/Details.stp?ID=160, doi: 10.3791/160 Abstract Analysis of gene expression patterns during early stages of mammalian embryonic development can provide important clues about gene function, cell-cell interaction and signaling mechanisms that guide embryonic patterning. However, dissection of the mouse embryo from the decidua shortly after implantation can be a challenging procedure, and detailed step-by-step documentation of this process is lacking. Here we demonstrate how post-implantation (6.5 dpc) embryos are isolated by first dissecting the uterus of a pregnant mouse (detection of the vaginal plug was designated day 0.5 poist coitum) and subsequently dissecting the embryo from maternal decidua. The dissection of Reichert'svaginal plug was designated day 0....
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- Spring '10
- RNA, Uterus, Journal of Visualized Experiments, Visualized Experiments