High-resolution magnetic resonance histology of the

High-resolution magnetic resonance histology of the -...

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High-resolution magnetic resonance histology of the embryonic and neonatal mouse: A 4D atlas and morphologic database Alexandra E. Petiet* , Matthew H. Kaufman , Matthew M. Goddeeris § , Jeffrey Brandenburg*, Susan A. Elmore , and G. Allan Johnson* i ** *Center for In Vivo Microscopy and Departments of § Cell Biology and i Radiology, Duke University Medical Center, Box 3302, Durham, NC 27710; Department of Biomedical Engineering, Duke University, Box 90281, Durham, NC 27708; Department of Biomedical Sciences, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh EH8 9XD, United Kingdom; and Laboratory of Experimental Pathology, National Institute of Envrionmental Health Sciences, Box 12233, Maildrop B3-06, 111 T.W. Alexander Drive, Research Triangle Park, NC 27709 Communicated by William Happer, Princeton University, Princeton, NJ, June 16, 2008 (received for review December 10, 2007) Engineered mice play an ever-increasing role in deFning connec- tions between genotype and phenotypic expression. The potential of magnetic resonance microscopy (MRM) for morphologic phe- notyping in the mouse has previously been demonstrated; how- ever, applications have been limited by long scan times, availability of the technology, and a foundation of normative data. This article describes an integrated environment for high-resolution study of normal, transgenic, and mutant mouse models at embryonic and neonatal stages. Three-dimensional images are shown at an iso- tropic resolution of 19.5 m m (voxel volumes of 8 pL), acquired in 3 h at embryonic days 10.5–19.5 (10 stages) and postnatal days 0–32 (6 stages). A web-accessible atlas encompassing this data was devel- oped, and for critical stages of embryonic development (prenatal days 14.5–18.5), > 200 anatomical structures have been identiFed and labeled. Also, matching optical histology and analysis tools are provided to compare multiple specimens at multiple developmen- tal stages. The utility of the approach is demonstrated in charac- terizing cardiac septal defects in conditional mutant embryos lacking the Smoothened receptor gene. ±inally, a collaborative paradigm is presented that allows sharing of data across the scientiFc community. This work makes magnetic resonance micros- copy of the mouse embryo and neonate broadly available with carefully annotated normative data and an extensive environment for collaborations. digital atlas u magnetic resonance microscopy u mouse embryo I n their seminal papers on MRI, Lauterbur (1) and Mansfield and Grannell (2) recognized the potential for magnetic reso- nance microscopy (MRM). Almost 13 years later, theory was reduced to practice with acquisition of the first MRI scans at microscopic spatial resolution (3–5). Some of the first applica- tions of MRM focused on the developing mouse embryo (6, 7).
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This note was uploaded on 05/28/2010 for the course BIOLOGY 03234 taught by Professor Sochacka during the Spring '10 term at Ghent University.

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High-resolution magnetic resonance histology of the -...

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