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Unformatted text preview: doi: 10.1136/jnnp.31.2.110 1968 31: 110-114 J Neurol Neurosurg Psychiatry S J Strich system. peripheral and central nervous staining degenerating myelin in the Notes on the Marchi method for http://jnnp.bmj.com/content/31/2/110.citation Updated information and services can be found at: These include: References http://jnnp.bmj.com/content/31/2/110.citation#related-urls Article cited in: service Email alerting online article. article. Sign up in the box at the top right corner of the Receive free email alerts when new articles cite this Notes http://jnnp.bmj.com/cgi/reprintform To order reprints of this article go to: http://jnnp.bmj.com/subscriptions go to: Journal of Neurology, Neurosurgery & Psychiatry To subscribe to group.bmj.com on February 17, 2010 - Published by jnnp.bmj.com Downloaded from J.Neurol.Neurosurg.Psychiat., 1968,31,110-114 Notes on theMarchimethodforstaining degeneratingmyelinintheperipheralandcentral nervous system SABINA J. STRICH From theDepartmentofNeuropathology,Institute of Psychiatry,London S.E.5 The Marchi method for staining degenerating myelin(Marchi, 1886)hasexasperatedandintrigued many becauseitissousefulyetpronetodisturbing artefacts and because the reaction of osmium tetroxide with tissue components has interesting theoreticalimplications (Adams, 1965). Essentially, tissuesareplacedinamixture ofosmium tetroxide andanoxidizingagentsuchasKC103; degenerating myelinthenbecomes black,whereasnormalsheaths remainunstained.Themethodhasbeenusedmainly to demonstrate so-called Wallerian degeneration: when a nerve fibre is interrupted, the axon and myelinsheathofthepieceseveredfromthecellbody degenerate and the breakdown products of this processcanbestainedwithMarchifluid. Therearecontradictoryviewsaboutthechemical nature of the material which reduces osmium tetroxideinthisprocedure.Thusthematerialwhich stainswiththeMarchimethodhasbeencharacterized as a lipid, probably cholesterol ester, by some (Adams, 1958; Wolfgram and Rose, 1958; Adams, 1960)and asa polysaccharideby others(Wolman, 1956, 1957). Conflicting opinions have also been expressedabout thepersistenceofthismaterial in the degeneratingneural tissue. Some workers state thatthemethodisonlyusefultwotofiveweeksafter theinterruptionofmyelinatednervefibres(Mettler and Hanada, 1942),whereas others have obtained satisfactory preparations from three days (Glees, 1948)tomanymonthsaftertheonsetofthedegenera- tion (Glees, 1943; Smith, 1956a). Similarly some authorities (Swank and Davenport, 1934; Mettler and Hanada, 1942; Glees, 1943) insist that the tissuetobestainedshouldnotbefixedformore than 48 hours, otherwise there may be lossofstainable material or increased artefacts; others claim good results,insomecases,yearsafterfixationinformalin (Smith,1956a;Smith,Strich,andSharp,1956)....
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This note was uploaded on 05/28/2010 for the course BIOLOGY 03234 taught by Professor Sochacka during the Spring '10 term at Ghent University.
- Spring '10