BIOSTATISTICS2 - LINKAGE and QTL MAPPING BIOSTATISTICS 2...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: LINKAGE and QTL MAPPING BIOSTATISTICS 2 academic year 2009-2010 Prof. Dr. ir. Marnik Vuylsteke Generation of Recombinant Inbred Lines (RILs) Broman, Genetics 2006 RIL mapping population marke r genotype for each RIL individual 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 1 B B A B B B B B B B A B A A A A B A A A B B A A B 2 B B B A A B B B B B A B A A A A B A A A A B A A B 3 B B B A A B B B B B A B A A A A B A A A A B A B B 4 B A B A A A B B B B A A A A A A B A A A A B A B B 5 B A B A A A B B B A A B A B A A B A A A A B B B B 6 A A B A A A B B B A A B A B A A B A A B A B B A A A= homozygous for parent A allele B = homozygous for parent B allele General form of a RIL design P 1 : AB/AB P 2 : ab/ab × F 1 : AB/ab ⇓ ... ⇓ F 8 AB/AB Ab/Ab aB/aB ab/ab (1-R)/2 R/2 R/2 (1-R)/2 ⇓ r-R relationship in a RIL design ¡ No one-to-one relationship anymore between R (recombinants) and r (recombination frequency) ¡ Calculating back to a single meiosis (Haldane and Waddington (1930)) ¡ example R = 0.16 ⇒ r = ± 0.1 ) 2 1 ( 2 )] 1 ( 2 /[ r r R R R r + = ⇔ − = r-R relationship in a RIL design 0.00 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.45 0.50 0.00 0.04 0.08 0.12 0.16 0.20 0.24 0.28 0.32 0.36 0.40 0.44 0.48 r R r-R relationship General form of a RIL design P 1 : AB/AB P 2 : ab/ab × F 1 : AB/ab ⇓ ... ⇓ F 8 AB/AB Ab/Ab aB/aB ab/ab 1/2(1+2 r ) r /(1+2 r ) r /(1+2 r ) 1/2(1+2 r ) AB/AB Ab/Ab aB/aB ab/ab (1-R)/2 R/2 R/2 (1-R)/2 ⇓ Estimation of r in a RIL design by the counting method Geno- type...
View Full Document

This note was uploaded on 05/28/2010 for the course WE BIBI010000 taught by Professor Marnikvuylsteke during the Spring '10 term at Ghent University.

Page1 / 20

BIOSTATISTICS2 - LINKAGE and QTL MAPPING BIOSTATISTICS 2...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online