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les_4_ppt_blackwhite - Work college 10/3(leszaal 4 Where...

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Work college 10/3 (leszaal 4) Where? Leszaal 4th floor from 14-18h • Topics: Problem solving by writing out experiments Interpretation of figures • ~exam questions! Presence is obliged • Bring along: your cursus + notes workcollege document (Minerva)
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Protein – protein interaction (PPI) Biological source material Living animals Generation of extract Protein RNA DNA 1 9,10,11 2 7,8 Protein 4,5,6 3 Cell death and proliferation techniques phosphorylation ubiquitination sumoylation acetylation methylation lipid modification ADP ribosylation Reporter systems and detection methods Epitope tagging, physical and gene-based protein-protein interaction methods: - Physical (affinity chromatography, TAP, pull down, coIP, affinity blotting, crosslinking, SPA, Biacore, protein arrays) -Gene-based (protein probing, phage display,2H and variants, FRET, FRAP, FLIP, BRET) Inhibition of gene expression: Antisense Ribozymes DNAzymes RNAinterference MicroRNA Tissue culture Course Gene technology
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Outline for lectures 4-6 1. Introduction 2. Epitope tagging 3. Biochemical approaches 1. in vitro interaction 2. Protein affinity chromatography 3. coIP 4. Crosslinking 5. TAP technology 6. SPA 7.
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