Work_college_I - 3 Stimulation time kinetics … 4 technique • 1 possibility • argument your choice 5 in vivo in vitro 6 read-out detection

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EMCB Work college I March 10 th 2009
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Intention of WC I • implement molecular cell biology techniques from EMCB lectures • How? 1. Problem solving by writing out experiments 2. Interpretation of figures or tables retrieved from articles or company brochures/websites ~exam questions! • Make use of your cursus + PPP
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Instruction • 19 groups of 2 students • Each group resolves 3 questions: – 2 questions of part 1 – 1 question of part 2 • Spare time: other questions • 1h preparation time
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Instruction • Both students of each group report orally on 1 question (15-18h) • Discussion: – Other groups can give their comments, their solution, fill the gaps, …
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Part 1 (Drexler et al., Arthritis Research and Therapy, 2008) Q1. IRF-3 is able to homodimerize upon TLR stimulation. Write down an experiment to check this interaction in mammalian cells.
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Part 1 Strategy step by step (cfr. in the lab), meaning: 1. Construct(s), transfection, … 2. -/+ controls
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Unformatted text preview: 3. Stimulation, time kinetics, … 4. technique • 1 possibility • argument your choice 5. in vivo / in vitro 6. read-out, detection Student A Student B Part 2 Interpretation of figures + data Questions: 1. which technique has been used? Interpret the figure, data (student A) 2. Propose an alternative strategy to evaluate the same question (student B) Group # - Q resolve Q: report on: student A student B group # part 1 part 2 part 1 part 2 1 1 2 1 1 2 3 4 2 3 3 5 6 3 5 4 7 8 4 7 5 9 10 5 9 6 1 2 6 2 7 3 4 7 4 8 5 6 8 6 9 7 8 9 8 10 9 10 1 10 11 1 2 2 1 12 3 4 3 2 13 5 6 4 3 14 7 8 5 4 15 9 10 6 5 16 1 2 7 6 17 3 4 8 7 18 5 6 9 8 19 7 8 1 9 • for part 1: student A reports on sub-questions 1 till 3 student B reports on sub-questions 4 till 6 • for part 2: student A reports on sub-question 1 student B reports on sub-question 2 1.1-3 1.4-7 1.8-10 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9...
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This note was uploaded on 05/28/2010 for the course WE BIBI000000 taught by Professor Johangrooten during the Spring '10 term at Ghent University.

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Work_college_I - 3 Stimulation time kinetics … 4 technique • 1 possibility • argument your choice 5 in vivo in vitro 6 read-out detection

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