Glycobiology_Course_lesson_2

Glycobiology_Course_lesson_2 - Glycobiology Course II....

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Glycobiology Session 2, slide 1 Glycobiology Course II. Biosynthesis Nico Callewaert
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Glycobiology Session 2, slide 2 Biosynthesis: enzymes 9 Glycosylation: enzymatic addition of oligosaccharides or monosaccharides to macromolecules •En z ym e s : ± Enzymes involved in Sugar activation ± Glycosyltransferases (Mannosyl-, fucosyl-, galactosyl-, …) – Transfer of activated sugars
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Glycobiology Session 2, slide 3 Biosynthesis: enzymes 9 Glycosylation: enzymatic addition of oligosaccharides or monosaccharides to macromolecules •En z ym e s : ± Enzymes involved in Sugar activation ± Glycosyltransferases (GT)(Mannosyl-, fucosyl-, galactosyl-, …) – Transfer of activated sugars – 91 families (2008)(www.cazy.org) ± Glycosidases or Glycosyl hydrolases (GH)(Mannosidase, fucosidase, galactosidase, …)
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Glycobiology Session 2, slide 4 Biosynthesis: enzymes 9 Sugar nucleotide synthesis: • Glycosyltransferases need activated sugars as donor substrates
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Glycobiology Session 2, slide 5 Biosynthesis: sugar nucleotides 9 Sugar nucleotide synthesis: • Monosaccharides from dietary sources, from endogenous conversions or from recycling and salvage pathways
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Glycobiology Session 2, slide 6 Biosynthesis: sugar nucleotides 9 Sugar nucleotide synthesis: • Monosaccharides from dietary sources, from endogenous conversions or from recycling and salvage pathways
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Glycobiology Session 2, slide 7 Biosynthesis: sugar nucleotides 9 Sugar nucleotide synthesis: : • Activation: ± Nucleoside mono- and diphophate sugars G1P UDP-Glucose
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Glycobiology Session 2, slide 8 Biosynthesis: sugar nucleotides 9 Sugar nucleotide synthesis: • Activation: ± Nucleoside mono- and diphophate sugars
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Glycobiology Session 2, slide 9 Biosynthesis: sugar nucleotides 9 Sugar nucleotide synthesis: • Activation: ± Nucleoside mono- and diphophate sugars
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Glycobiology Session 2, slide 10 Biosynthesis: sugar nucleotides 9 Sugar nucleotide synthesis: • Activation: ± Nucleoside mono- and diphophate sugars –GD P -M a n –GD P -
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Glycobiology Session 2, slide 11 Biosynthesis: sugar nucleotides 9 Sugar nucleotide synthesis: • Interfering with glycosylation: putting ‘handles’ on a cell
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Glycobiology Session 2, slide 12 Biosynthesis: sugar nucleotides 9 Sugar nucleotide transport: • Synthesis of sugar nucleotides: cytosol/nucleus (CMP-NANA) Glycosylation: ER and Golgi • Active transport from cytosol to organelle • Transporters = antiporters: ± Antiport with cognate nucleotide byproducts of glycosylation reaction ± Hydrophobic proteins with multiple membrane spanning domains
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Biosynthesis: sugar nucleotides
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This note was uploaded on 05/28/2010 for the course WE BIBI000000 taught by Professor Johangrooten during the Spring '10 term at Ghent University.

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Glycobiology_Course_lesson_2 - Glycobiology Course II....

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