Glycobiology_Course_lesson_2

Glycobiology_Course_lesson_2 - Glycobiology Course I I ....

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Glycobiology Session 2, slide 1 Glycobiology Course II. Biosynthesis Roland Contreras Nico Callewaert Wouter Laroy
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Glycobiology Session 2, slide 2 Biosynthesis: enzymes Glycosylation: enzymatic addition of oligosaccharides or monosaccharides to macromolecules Enzymes: Enzymes involved in Sugar activation Glycosyltransferases (Mannosyl-, fucosyl-, galactosyl-, …) – Transfer of activated sugars Glycosidases (Mannosidase, fucosidase, galactosidase, …) – Hydrolysis of sugar linkages Others (OST, phosphotransferases, sulphotransferases, …)
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Glycobiology Session 2, slide 3 Biosynthesis: enzymes Glycosylation: enzymatic addition of oligosaccharides or monosaccharides to macromolecules Enzymes: Enzymes involved in Sugar activation Glycosyltransferases (GT)(Mannosyl-, fucosyl-, galactosyl-, …) – Transfer of activated sugars – 52 families (2001)(http://afmb.cnrs-mrs.fr/CAZY/families.html) Glycosidases or Glycosyl hydrolases (GH)(Mannosidase, fucosidase, galactosidase, …) – Hydrolysis of sugar linkages – 85 families (2001) Others (OST, phosphotransferases, sulphotransferases, epimerases…)
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Glycobiology Session 2, slide 4 Biosynthesis: enzymes Sugar nucleotide synthesis: Glycosyltransferases need activated sugars as donor substrates
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Glycobiology Session 2, slide 5 Biosynthesis: sugar nucleotides Sugar nucleotide synthesis: Monosaccharides from dietary sources, from endogenous conversions or from recycling and salvage pathways
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Glycobiology Session 2, slide 6 Biosynthesis: sugar nucleotides Sugar nucleotide synthesis: Monosaccharides from dietary sources, from endogenous conversions or from recycling and salvage pathways
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Glycobiology Session 2, slide 7 Biosynthesis: sugar nucleotides Sugar nucleotide synthesis: : Activation: Nucleoside mono- and diphophate sugars G1P UDP-Glucose
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Glycobiology Session 2, slide 8 Biosynthesis: sugar nucleotides Sugar nucleotide synthesis: Activation: Nucleoside mono- and diphophate sugars
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Glycobiology Session 2, slide 9 Biosynthesis: sugar nucleotides Sugar nucleotide synthesis: Activation: Nucleoside mono- and diphophate sugars
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Glycobiology Session 2, slide 10 Biosynthesis: sugar nucleotides Sugar nucleotide synthesis: Activation: Nucleoside mono- and diphophate sugars – GDP-Man – GDP-Fuc – UDP-Glc – UDP-Gal – UDP-GlcNAc – UDP-GalNAc – CMP-NANA – …
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Glycobiology Session 2, slide 11 Biosynthesis: sugar nucleotides Sugar nucleotide synthesis: Interfering with glycosylation: putting ‘handles’ on a cell
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Glycobiology Session 2, slide 12 Biosynthesis: sugar nucleotides Sugar nucleotide transport: Synthesis of sugar nucleotides: cytosol/nucleus (CMP-NANA) Glycosylation: ER and Golgi Active transport from cytosol to organelle Transporters = antiporters: Antiport with cognate nucleotide byproducts of glycosylation reaction Hydrophobic proteins with multiple membrane spanning domains
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This note was uploaded on 05/28/2010 for the course WE BIBI000000 taught by Professor Johangrooten during the Spring '10 term at Ghent University.

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Glycobiology_Course_lesson_2 - Glycobiology Course I I ....

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