In-vivo_bio-imaging-2009-1

In-vivo_bio-imaging-2009-1 - In-vivo bio-imaging2009...

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Unformatted text preview: In-vivo bio-imaging2009 2ndsemesterdr. ir. LauryChaerleOverview / Scope•Thermal imaging•Chlorophyll fluorescence imaging•Reflectance imaging•Near infrared imaging•Hyperspectral imaging•UV-induced fluorescence imaging•Bioluminescence imaging•Magnetic resonance imagingCourse scheduleMinervaadditional infobasic image processing courseCourse objectives•From remote sensing to microscopywhich techniques at what application scale•Main areas of applicationsfunctional analysis of plant genes -breedingprecision farming•Plant physiologicalprocessesvisualisedcomplementarityof techniquesscreeningCourse overview•8 main types of imaging techniquesapplications illustrated by key publications•Imaging-guided sampling histochemicalor molecular characterisationtargeted analysis•Image acquisition Æmonitoringprocessing and quantitative analysisImaging for early stress-detectionThermographyFluorescence imagingChlorophyll fluorescenceUV-induced fluorescenceGFP imagingBioluminescence imagingaequorinluciferaseReflectance imagingHyperspectral imagingNMRNon-destructiveNon-contactModalities10 μmTechnical Overview•Reflectance imagingvisual spectrum detection CCD / CMOSUVNIR red edgestructural characteristicsilluminationangle of image captureHyperspectral wavelength bandsmetabolite monitoringTechnical Overview•Thermal imagingMaterials with high electron mobilityScanning systemsMimic naturefire beetlepit viperMicrobolometersObject emissivityblackbodyThermography Setup8-12μm spectral range thermal cameraNobel, P.S. 199125cmThermal DetectorScanner : point detectorFPA : bolometer arrayresolution 0.1degree5cmSkubatzH., Nelson T.A., Dong A.M., MeeuseB.J.D. & BendichA.J. (1990) Infrared thermography of Arum lily inflorescences. Planta 182, 432-436.ThermogenicplantsFlower temperature 10-20°C above ambientThermography in plant physiology Voodoo lily Sauromatum guttatum (Araceae) Raskinet al. 1989ranspirationMajor factor in energy budget of plant leavesSR + LR + C + E (+ M) = 0Short wave radiationLong wave radiationConvec-tionTranspi-rationMetabo-lismLambers, Chapin & Pons 1998Thermography and plant temperatureetabolic burstthermogenicplantshermal measurementsNon-contact temperature sensorsinfrared thermocouplethermal cameraFieldGrowth chamberControlled measuring cabinetThermography & leaf temperature...
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This note was uploaded on 05/28/2010 for the course WE BIOL000000 taught by Professor Laurychaerle during the Spring '10 term at Ghent University.

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In-vivo_bio-imaging-2009-1 - In-vivo bio-imaging2009...

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