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In-vivo_Bio-imaging_Seminar-Roland-Valcke_17032009

In-vivo_Bio-imaging_Seminar-Roland-Valcke_17032009 -...

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Fluores cence as a tool to communicate with plants: an example of vitality monitoring Roland Valcke Hasselt University, Diepenbeek UGent, 17/03/2009 Bio-imaging
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Fluores cence as a tool to communicate with plants Outline : - introduction : something about photosynthesis - fluorescence : Î what is it? Î where does it come from? Î what can you do with it Î fluorescence induction and quenching - fluores cence imaging : Î experimental set-up, prototypes Î ‘applications’, ‘combinations’, LIF (a selection) - some conclusions , potentialities and limitations
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Photosynthesis Life on earth is solar-powered. The chloroplasts of plants capture light energy that has traveled 160 million kilometers from the sun and convert it to chemical energy stored in sugar and other molecules. The process is called photosynthesis . Plants Cyanobacteria Photosynthetic bacteria h ν inorganic products organic products 6 CO 2 + 12 H 2 O Æ C 6 H 12 O 6 + 6 O 2 + 6 H 2 O
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Photosynthesis in higher plants, tissue most active in photosynthesis: leaf mesophyl cells: chloroplasts with chlorofyl + other pigments: carotenoïds light-energy used for: oxidation of water Î release of oxygen synthesis of carbon components, sugars (+ ……..!!) light-reactions in thylakoïd membranes ( photosystems ): final products: ATP + NADPH carbon-fixation reactions: synthesis of sugars in stroma (Calvin-Benson cyclus)
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