A93674F1d01 - Systems Biology - Interactomics - Jelle Van...

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Unformatted text preview: Systems Biology - Interactomics - Jelle Van Leene 12/4/2009 Leene Academic Year 2009-2010 1 Introduction on interactomic Introduction on interactomics Node protein Node = protein Edge = interaction 1 What? Interactomics is a discipline of bioinformatics and biology that deals with studying both the interaction biology that deals with studying both the interactions and the consequences of those interactions between and among proteins , and other molecules within a cell (e g protein DNA (e.g. protein-DNA) Why? Proteins, encoded on DNA, do the actual job. They do not work alone , but they form protein complexes = molecular machines 2 Systems Biology - Interactomics - Jelle Van Leene 12/4/2009 Leene Academic Year 2009-2010 2 Components Machines Circuits Proteins Organisms Networks 3 Detection of protein-protein interactions • Genetic approaches: Y2H • Y2H • MAPPIT (in mammalian cells) • Protein-fragment Complementation Assay (DHFR β lactamase spli luciferase (DHFR, β-lactamase, split-luciferase) • FRET/BRET Allow subcellular localization of • Split-GFP interaction 4 Systems Biology - Interactomics - Jelle Van Leene 12/4/2009 Leene Academic Year 2009-2010 3 Detection of...
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This note was uploaded on 05/28/2010 for the course WE BIBI010000 taught by Professor Marnikvuylsteke during the Spring '10 term at Ghent University.

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A93674F1d01 - Systems Biology - Interactomics - Jelle Van...

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