C13BFADEd01 - ANNUAL REVIEWS Further Click here for quick...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Systematic Mapping of Genetic Interaction Networks Scott J. Dixon, 1 , 2 , Michael Costanzo, 1 , Anastasia Baryshnikova, 1 Brenda Andrews, 1 and Charles Boone 1 1 Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 1A7, Canada; email: [email protected] 2 Department of Biological Sciences, Columbia University, New York, New York 10027 Annu. Rev. Genet. 2009. 43:601–625 First published online as a Review in Advance on August 27, 2009 The Annual Review of Genetics is online at genet.annualreviews.org This article’s doi: 10.1146/annurev.genet.39.073003.114751 Copyright c ° 2009 by Annual Reviews. All rights reserved 0066-4197/09/1201-0601$20.00 These authors contributed equally to this work. Key Words genetic interaction, network, synthetic lethal, Saccharomyces cerevisiae , epistasis Abstract Genetic interactions influencing a phenotype of interest can be identi- ±ed systematically using libraries of genetic tools that perturb biological systems in a de±ned manner. Systematic screens conducted in the yeast Saccharomyces cerevisiae have identi±ed thousands of genetic interactions and provided insight into the global structure of biological networks. Techniques enabling systematic genetic interaction mapping have been extended to other single-celled organisms, the bacteria Escherichia coli and the yeast Schizosaccharomyces pombe , opening the way to comparative investigations of interaction networks. Genetic interaction screens in Caenorhabditis elegans , Drosophila melanogaster, and mammalian models are helping to improve our understanding of metazoan-speci±c signal- ing pathways. Together, our emerging knowledge of the genetic wiring diagrams of eukaryotic and prokaryotic cells is providing a new under- standing of the relationship between genotype and phenotype. 601 Annu. Rev. Genet. 2009.43:601-625. Downloaded from arjournals.annualreviews.org by RIJKSUNIVERSITEIT GENT on 12/04/09. For personal use only.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
INTRODUCTION A genetic interaction refers to an unexpected phenotype not easily explained by combining the effects of individual genetic variants (7). Genetic interactions are thought to underlie di- verse biological phenomena such as the evolu- tion of sex, speciation, and complex disease (1, 11, 67, 121). At the level of an individual or- ganism, understanding how genes interact with one another to produce a given phenotype is a challenge of immense signiFcance to basic bi- ologists and clinicians alike. Despite recent ad- vances (16, 17), mapping genetic interactions within individuals from outbred populations remains a difFcult task. Researchers have there- fore embraced inbred model systems, such as yeast and worm, as well as isogenic populations of cultured cells derived from fruit flies and mammals, as platforms to map genetic inter- actions in a systematic, unbiased, and compre- hensive fashion (13, 53). Compared with classic forward genetic modiFer screens, which typi- cally focus on the identiFcation of a small num- ber of second-site mutations, systematic reverse
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 05/28/2010 for the course WE BIBI010000 taught by Professor Marnikvuylsteke during the Spring '10 term at Ghent University.

Page1 / 27

C13BFADEd01 - ANNUAL REVIEWS Further Click here for quick...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online