les2 - Transgenetics of Animal Model Organisms Partim...

Info iconThis preview shows pages 1–17. Sign up to view the full content.

View Full Document Right Arrow Icon
Transgenetics of Animal Model Organisms Partim Mammalia: Libert Rest: Vleminckx
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Part 1:Introduction Movie Chapter I: The mouse as an experimental animal Chapter II: Wild mice and laboratory mice Chapter III: Reproduction and breeding Chapter IV: The mouse genome
Background image of page 2
Part 1: Chapter 1: The mouse as an experimental animal
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
1.1. Experimental Animals definition : - experimental animals (medical research, developmental biology, . ..) - bioreactors, meat production, organs,… - ethically: only adults - here: animals that undergo transgenesis mammals scientific research biotechnological products
Background image of page 4
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
literature : ‘transgenesis’ of mammals ± exclusively mouse - techniques for isolating, manipulating of germ cells and embryo’s, ES cells - vaginal plug timing / age embryo’s - injection of DNA in zygote possible - embryo’s 2-4-8-32 : cells exchangeable chimera’s - nucleus transplantation - normal ovulation : 6-10 or more oöcytes - superovulation possible - oestrus cycle of only 4 days. First ovulation at age of 4w – 8w
Background image of page 6
- gestation : 3 w generation time 10-12 w (1 couple leads to 250 offspring/year) - small : 25-35 g important for animal house - males do not harm pups (in contrast to rats) - docile and easy to manipulate - excellent model for physiol. aspects man pathol. : mimicking diseases such as arthritis, lupus, IBD, schizophrenia… - long history as an exp animal : lot of data present - genetic map dense/genome sequenced + choice of mouse as mammalian model organism (rather by coincidence)
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
- Disadvantages mouse: size, physiology, not little humans,… - this course ± exclusively dedicated to mouse - but also some aspects of rat, rabbit, sheep - essential characteristics : figure
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
1.2. Relation mouse-man Micky mouse : illustrates close relation - since ± 10.000 year (late neolithicum, after last ice age) - humans change from nomadic to sedentary - starts in Mesopotamia / Bactria (Pakistan) coincidentially there ancestors of house mouse
Background image of page 10
- sedentary existence food stocks paradise for mouse - spreading starting from Pakistan ( cf. figure ) - now : house mice everywhere (Antarctica, Himalaya, space shuttle,. ..)
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 12
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
- muis / mouse/ mulôt > Mus > Mug > Mush = to steal souris - cat - 19 th C : kitsch : Japan / China/ USA shops with variants : creamy buff red cream ruby-eye-yellow
Background image of page 14
- beginning 20th C : - Miss Abbie Lathrop : * thousands breeding couples * professional / systematical * close to Harvard . ....... - Rediscovery laws of Mendel 1. Cuénot 1902 : laws not only for plants 2. Castle & Little (Harvard) : inbreeding gen. homozygote animals DBA
Background image of page 15

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
forbade Gregor Mendel to study inheritance of coat color traits of mice, mouse genetics may have had its beginnings in 1866 instead of 1902. The bishop in Mendel’s district felt it was inappropriate for the monk to share his living quarters with critters that had sexual intercourse, and so Mendel was forced to turn his attention to making experimental crosses with garden pea. Fortunately for Mendel, the bishop was apparently unaware that plants also had sex”
Background image of page 16
Image of page 17
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 05/28/2010 for the course WE BIBI000000 taught by Professor Johangrooten during the Spring '10 term at Ghent University.

Page1 / 77

les2 - Transgenetics of Animal Model Organisms Partim...

This preview shows document pages 1 - 17. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online