{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

3A430295d01 - Systems Biology Systems Biology Synthetic...

Info icon This preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
Systems Biology 1 Academic year 2009 - 2010 Systems Biology Synthetic Synthetic biology biology Synthetic Genetic networks Prof. Dr Prof. Dr. Pierre Hilson . Pierre Hilson VIB Department of Plant Systems Biology University of Ghent Academic year Academic year 2009 2009-2010 2010 Outline - Introduction Synthetic genetic networks - Synthetic genetic networks - Building biological systems as engineers Building biological systems as engineers
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Systems Biology 2 Academic year 2009 - 2010 I t d ti Introduction ¤ The engineering of biology: the synthesis of complex, biologically based systems, which display novel functions that do not exist in nature ¤ May be applied at all levels of the hierarchy of biological ¤ May be applied at all levels of the hierarchy of biological structures ¤ C l it b tl th h th i i l f ¤ Complexity may be partly overcome through the principles of hierarchical abstraction, modularity, standardization and flexibility, and by defining appropriate levels of abstraction to describe and design biological systems (cf. microelectronics) ¤ SB enables large-scale production/conversion of materials – E g redesigned metabolic pathways for pollution remediation and E.g. redesigned metabolic pathways for pollution remediation and for synthesis of drugs, plastics and nutriments ¤ G d i i ht t l f ti id th ti ¤ Genome design might create novel function or widen the genetic code to provide flexibility unattainable in natural cells Outline - Introduction Synthetic genetic networks - Synthetic genetic networks - Building biological systems as engineers Building biological systems as engineers
Image of page 2
Systems Biology 3 Academic year 2009 - 2010 N t k tif Network motifs Regulators Promoters Binding Link of 2 reg. Yields testable models Lee et al (2002) Science 298: 799 S th ti bi l t Synthetic biology concept Kobayashi ey al. (2004) PNAS 101, 8414
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Systems Biology 4 Academic year 2009 - 2010 A synthetic oscillatory network of transcriptional regulators Elowitz and Leibner (2000) Nature 403: 335 ¤ What are the “design principles” underlying the intracellular ¤ What are the design principles underlying the intracellular networks Th d i d i f h i k i h ¤ The design and construction of a synthetic network with a particular function should help understand natural circuits Th il t / t hit t The repressilator/reporter architecture repressor from Tn 10 repressor from λ repressor from E. coli from lite: repressors include destruction tags cyclic negative feedback loop Elowitz and Leibner (2000) Nature 403: 335
Image of page 4
Systems Biology 5 Academic year 2009 - 2010 M th ti l d li (1) Mathematical modeling (1) ¤ Goals Description in sufficient details to identify possible classes of dynamic behavior Identification of key adjustable experimental parameters ¤ Molecular interactions cannot be described exhaustively ¤ The model takes into consideration protein and corresponding mRNA The model takes into consideration protein and corresponding mRNA concentration as continuous dynamical variables ¤ The molecules participate in transcription, translation and degradation.
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern