AF7706F7d01 - Bottom-up modeling II Multicellular phenomena...

Info iconThis preview shows pages 1–13. Sign up to view the full content.

View Full Document Right Arrow Icon
Bottom-up modeling II Multicellular phenomena Roeland Merks Netherlands Institute for Systems Biology Amsterdam http://www.sysbio.nl and http://www.cwi.nl Systems Biology October 23rd, 2009
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Biological development DNA + fertilized egg cell -> 3D shape + function Movie: Zebrafish development, P.Z. Myers, University of Minnesota (source: YouTube) Zebrafish Wikipedia
Background image of page 2
Individual cells seemingly act independently. .. Zebrafish blastoderm (embryonic tissue), P.Z. Myers, University of Minnesota (source: YouTube)
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
... but cells respond to each other Contact-inhibition in cultured frog neural crest cells. Carmona-Fontaine et al. Nature 2008.
Background image of page 4
How does genetics regulate development? 1. How does genetics regulate cell behaviors? 2. How do collective cell behaviors follow from behaviors of individual cells ?
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
How does genetics regulate development? 1. How does genetics regulate cell behaviors? 2. How do collective cell behaviors follow from behaviors of individual cells ?
Background image of page 6
How does genetics regulate development? 1. How does genetics regulate cell behaviors? 2. How do collective cell behaviors follow from behaviors of individual cells ? • Experiments in cell cultures and in vivo • Cell-based, computational modeling
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
So development is not just genetics! How does the pattern of gene expression act through physical and chemical mechanisms to result in the structures we observe? Genetics is the beginning Most plant or mammalian cells are fairly limited in their behaviors. They can: Grow, divide, change shape, move spontaneously, move in response to external cues, stick, absorb and secrete chemical signals, exert forces, respond to mechanical signals, adhere to other cells, and change their adhesion properties, send and respond to electrical signals, etc So: complex pathways often have “simple” results
Background image of page 8
Cell-based Modeling See e.g. Merks et al. Phys. A 2005 Genetic and metabolic networks primarily regulate individual cells – Response to extracellular signals, secretion of signaling and extracellular matrix proteins, cell migration, cell adhesion, etc. To understand how genetics regulates multicellular phenomena, we must ask: – how genetics drives cell behavior ( i.e. networks) – how cell behavior produces multicellular patterns – how the cells (and their regulatory networks) respond to the multicellular environment “Middle-out approach” (Brenner, cited in Denis Noble 2006, The Music of Life) – the cell in the middle
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Differential adhesion hypothesis Steinberg 1963, Science 141, 401-408
Background image of page 10
Differential adhesion – Cell sorting James Glazier
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Graner and Glazier, 1992 Cellular automata model: cells on a grid • A patch of CA sites of the same state represents biological cells • Energy minimization formalism: – Generalized energy represents system state: cell size, cell adhesion, chemotaxis, etc. – Cells attempt to copy their state into
Background image of page 12
Image of page 13
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 05/30/2010 for the course WE CMBIBI0200 taught by Professor Pierrehilson during the Spring '10 term at Ghent University.

Page1 / 88

AF7706F7d01 - Bottom-up modeling II Multicellular phenomena...

This preview shows document pages 1 - 13. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online