842F3935d01 - Practical College: Transgenesis in laboratory...

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Practical College: Transgenesis in laboratory animals Partim Prof Dr. C. Libert Tue 30 and wed 31 march 2010 Tino Hochepied (Tino.Hochepied@dmbr.VIB-Ugent.be) Jinke D’Hont (Jinked@dmbr.VIB-Ugent.be)
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1. Overview of exercises and demos Demos + exercises Dissectionof oviductfor isolation of oocytes Dissectionof oviduct + small part of the uterus for isolation of morulas Dissectionof oviduct + complete uterus for isolation of blastocysts Isolation of oocytes + removal of cumulus cells with hyaluronidase (Flushing of the oviduct through the infundibulum for isolation of morulas) only if there is some time left Removing zona pellucida from morulas with Tyrode’s acid Aggregation of the morulas with ES-cell clumps Flushing of the uterus for isolation of blastocysts Demos only Oviduct transfer Uterus transfer
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2. Explanation of the use of the different techniques = to increase the number of oocytes that are ovulated PMSG = gonadotropin from pregnant mare’s serum mimicks follicle stimulating effect of endogenous Follicle Stimulating Hormone hCG = human chorionic gonadotropin mimicks ovulation inducing effect of endogenous Lutenizing
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This note was uploaded on 05/30/2010 for the course WE CHBIBI0000 taught by Professor Krisvleminckx during the Spring '10 term at Ghent University.

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842F3935d01 - Practical College: Transgenesis in laboratory...

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