830245FBd01 - Transgenetics of Animal Model Organisms...

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Transgenetics of Animal Model Organisms
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Course: 1. two parts Partim Mammalia: Libert Rest (C. elegans, Drosophila, zebrafish, Xenopus) : Vleminckx 2. Lessons Mammalia 8/2: I,II 22/2: III, IV 1/3: V, VI 8/3: VII, VIII 15/3: IX, X 22/3: XI, XII?
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3. Work colleges 9/2: cancelled 23/3 : postponed 1 week (switched with Prof. Savides) 30/3: Work college > two groups > divide group 31/3 4/5: WC Vleminckx 4. Course notes/syllabus 5. Problems Claude.Libert@Ugent.be 09/3313700 Technologiepark 927 (VIB building)
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Aims: 1.To learn the students about technology for genetic manipulation of model organisms 2. Overview of available research tools 3. In a historical perspective 4. Understanding much more important than reproducing matter 5. Exam is combination of theory and exercise 6. Problems: contact teacher
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Part 1:Introduction Movie Chapter I: The mouse as an experimental animal Chapter II: Wild mice and laboratory mice Chapter III: Reproduction and breeding Chapter IV: The mouse genome
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Part 1: Chapter 1: The mouse as an experimental animal
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1.1. Experimental Animals definition : - experimental animals (medical research, developmental biology, . ..) - bioreactors, meat production, organs,… - ethically: only adults - here: animals that undergo transgenesis mammals scientific research biotechnological products
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literature : ‘transgenesis’ of mammals ± exclusively mouse - techniques for isolating, manipulating of germ cells and embryo’s, ES cells - vaginal plug timing / age embryo’s - injection of DNA in zygote possible - embryo’s 2-4-8-32 : cells exchangeable chimera’s - nucleus transplantation - normal ovulation : 6-10 or more oöcytes - superovulation possible - oestrus cycle of only 4 days. First ovulation at age of 4w – 8w
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- gestation : 3 w generation time 10-12 w (1 couple leads to 250 offspring/year) - small : 25-35 g important for animal house - males do not harm pups (in contrast to rats) - docile and easy to manipulate - excellent model for physiol. aspects man pathol. : mimicking diseases such as arthritis, lupus, IBD, schizophrenia… - long history as an exp animal : lot of data present - genetic map dense/genome sequenced + choice of mouse as mammalian model organism (rather by coincidence)
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- Disadvantages mouse: size, physiology, not little humans,… - this course ± exclusively dedicated to mouse - but also some aspects of rat, rabbit, sheep - essential characteristics : figure
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1.2. Relation mouse-man Micky mouse : illustrates close relation -since ± 10.000 year (late neolithicum, after last ice age) - humans change from nomadic to sedentary - starts in Mesopotamia / Bactria (Pakistan) coincidentially there ancestors of house mouse
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