Bio 142 Back Exam Set 2

Bio 142 Back Exam Set 2 - Practice Exam III Bio 142 Spring...

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Practice Exam III Bio 142 Spring 2009 Name___________________________________ MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) Semiconservative replication involves a template. What is the template? A) DNA polymerase contains the template needed. B) an RNA molecule C) one strand of the DNA molecule D) single-stranded binding proteins E) chromosomal proteins 2) Put the following steps of DNA replication in chronological order. 1. Single-stranded binding proteins attach to DNA strands. 2. hydrogen bonds between base pairs of antiparallel strands are broken. 3. Primase binds to the site of origin. 4. DNA polymerase binds to the template strand. 5. An RNA primer is created. A) 3, 2, 1, 5, 4 B) 1, 2, 3, 4, 5 C) 2, 1, 3, 5, 4 D) 1, 2, 3, 4, 4 E) 4, 3, 2, 1, 5 Figure 14.2 3) Identify the lagging strand in Figure 14.2. A) a B) b C) c D) d 4) What is a major difference between eukaryotic DNA replication and prokaryotic DNA replication? A) DNA polymerase III of eukaryotes has both endonuclease and exonuclease activity, while that of prokaryotes has only exonuclease activity. B) Prokaryotic replication does not require a primer. C) Prokaryotic chromosomes have a single origin of replication, while eukaryotic chromosomes have multiple origins of replication. D) DNA polymerases of prokaryotes can add nucleotides to both 3 ! and 5 ! ends of DNA strands, while those of eukaryotes function only in the 5 !" 3 ! direction. E) none of the above
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Figure 14.3 5) Refer to Figure 14.3. Which of the structures in the figure breaks hydrogen bonds between complementary bases? A) DNA polymerase B) Helicase C) DNA ligase D) sliding clamp E) single stranded binding protein 6) Over expressing telomerase can be used to extend the life span of normal human cells. Telomere shortening puts a limit on the number of times a cell can divide. How might adding telomerase affect cellular aging? A) Telomerase would have no effect on cellular aging. B) Telomerase shortens telomeres and thus delays cellular aging. C) Telomerase will speed up the rate of cell proliferation. D) Telomerase ensures that the ends of the chromosomes are accurately replicated and eliminates telomere shortening. E) none of the above 7) DNA replication is highly accurate. It results in about one mistake per billion nucleotides. For the human genome, how often would errors occur? A) on average, once every 6 cell divisions B) on average, once or twice in the lifetime of an individual C) on average, once a lifetime in 10% of the population D) on average, once each time the entire genome of a cell is replicated E) on average, 6 times each time the entire genome of a cell is replicated 8) Researchers found E. coli that had mutation rates 100 times higher than normal. What is a possible explanation for these results? A) There were one or more mismatches in the RNA primer.
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This note was uploaded on 05/29/2010 for the course BIOL 142 taught by Professor Spell during the Spring '07 term at Emory.

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Bio 142 Back Exam Set 2 - Practice Exam III Bio 142 Spring...

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