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Unformatted text preview: Biochem lec 12.1 2 major modular cascade 1- the MAP kinase cascade 2- the PIP3 K c- insulin rece - the IRS 1, 2, 4 are activated after they become recruited o and they recruited to the PTyr by there SH2 domain o and the SH2 domains recognize PTyr residue on the chain in the cytoplamsic tail of the insulin rece - how the IRS phosphorelated on this Tyr ???? how os responsible for this P ??? it is the insulin rece K o and this mean GRB can be recruited - and then SOS is activated o which make the exchange of the GDP with GTP in Ras this is all happen in the plasma mem of the insulin responsive cell - and then you get an active Ras Raf MEK MAP K enter the nuc and phosphorlate a TF and transcribtion of gene induced The PTyr residue then recruite via the SH2 domain the adaptor molecule PIP3 K is activated PDGF is unlike insulin GF : cant only activate PIP 3 K and MAP K but also PL both the insulin rece and the PDGF can activate PIP3 K - PIP 3 K is recruited to the PDGF rece - Where in the insulin it is activated via IRS in aPTyr relation The PIP 3 K is tow subunit : the catalytic and the regulatory subunit The PIP 3 K to be recruited ????The PIP 3 K to be recruited ?...
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- Spring '10