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bio lec 10.11 - Mon 10.11 The Golgi network consists of a...

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Mon 10.11 The Golgi network consists of a stack of vesicular tubules The Golgi apparatus(GA) - consists of a system of membrane enclosed compartments (A) . - The GA is a stack of compartments with 4-6 cisternae (however, some flagelates contain up to 60). - In animal cells the cisternae are connected by tubules and - are found near the nucleus (B). - The GA has two distinct faces the cis Golgi- network (CGN) and the trans Golgi- network(TGN). o CGN is the entry site of the GA, o TGN the exit site. ligosaccharid side chains are processed in the Golgi Electron microscopy of the Golgi apparatus - (A) unstained revealing compartmentalization. - (B) Staining with osmium, which is preferentially reduced in the cis-cisterna. - (C) Nucleoside diphosphataseis located in the trans- cisterna, - whereas (D ) acid hydrolaseis found in the trans-Golgi network. 1
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Oligosaccharid side chains are processed in the Golgi Oligosaccharide chains are processed within the lumen of the GA. It appears that the proteins experience different glycosylations and modifications in specific cisternae of the golgi stack (B).
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