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bio lec -10-11 - Bio done by Huda the first part 1 20 after...

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Bio done by Huda the first part 1- 20 after me masha3el 10-11-2008 the transport process; the process going on b/w the ER on route to Golgi apparatus, how this process are done, how these are getting specifically managed in a cell? the G ptn again play an important role for this process to buid up a COPII coated vesicle for instance, these COPII coated vesicle are similar to the clathrin vesicle but they will contain other ptn of the surface of the vesicle, These vesicle need these structure because they contain specific material and this is the kind of address which the vesicle needs to low specific cargo into the lumen of the vesicle and to transport the vesicle to the certain destination. So to manage that G-ptn we have learned also very important because for the cell of course it important to know where from this ER patch and these formation of these coat ptn, and the vesicle has to be formed. And once striking mechanism - the GEF and Sar1-GEF which is catalyzing the exchange of the GDP instead of the for GTP at this small G ptn. - And then what we have see here is an amphipathic helix is move out of molecule and having to anchor This ptn on the outer leaflet of the outer membrane. - So the conformational change which is induced by exchange GDP against GTP leads to changes of the property of this molecule which have to anchor this here. - And then this is used to collect other ptn on the surface and these coat ptn are also recognizing the cytoplasmic tail of cargo receptor which fixes again on the inner part a certain cargo and 1
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- then a specific process in which the cell can manage to transport a certain cargo to a certain destination. - So then we have seen that this curvature in the membrane and this is induced by forming this patch and there other ptn also necessary and this is a cooperative step I - told you the Phosphoinozetol and the recognition of the phosphinosetile to the certain membrane are also very important for the formation and the assembly of all these ptn on the surface and then the vesicle is formed. So what we have also seen has G ptn are also necessary when the vesicle already formed, now we have the task how gets the vesicle to certain destination, how do we know where this vesicle belong to formed? - this family here the Rab-GTPase they comprise one of the largest family of small G ptn and then this G ptn had to specifically identify target membrane So how does Rab-GTP help us to transport the ptn into the target membrane. - here is the cargo receptor, - the cargo again - the v-SNARE and the - Rab-GTP, 1. the Rab-GTP is recognize by some Rab effector ptn on the a surface of this target receptor so called ( tethering receptor ) which is like a fishing line go into the cytosol and they recognize by Rab- GTP and in this way this vesicle is fixed to certain membrane, 2.
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