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Unformatted text preview: b. Identify the term (x- or y-intercept, or slope) with K I Example: Mixed inhibition, where K I does not equal K I ' 1) To determine K I , use the slope = (1 + [I]/Ki) Km/Vmax Solve for K I---> K I = [I]/[(Vmax/Km) slope - 1)] 2) To determine K I ', use the y-intercept = (1 + [I]/K I ')/Vmax Solve for KI' ---> KI' = [I]/[(Vmax x y-intercept) –1] 3) Therefore, the Km, Vmax, slope of the line for one of the inhibitor concentrations can be used to determine K I , and the Vmax, y-int. for one of the inhibitor concentrations can be used to determine K I '. c. Obtain expressions for the K I for competitive and uncompetitive inhibition. The following data were obtained on isocitrate lyase from an algae species. Deduce the Km and Vmax for the enzyme and the type of inhibition, and determine the Ki for the inhibitor.-20-10 10 20 30 40 50 100 150 200 250 1/[S], mM-1 1/v, μ M-1 min...
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