Key+Assignment+1

Key+Assignment+1 - BIS 104, Section 1 Spring Quarter, 2009...

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BIS 104, Section 1 Spring Quarter, 2009 Key to Assignment 1 1. The fact that the protein was extracted from the membrane with detergent and contains 3 α -helical segments suggests that it is an integral transmembrane protein that makes 3 passes through the membrane. The ability to label the tyrosines at positions 703 and 757 shows that the COOH- terminal portion of the protein is present on the exterior of the membrane. The inability to label the other tyrosines sows that these tyrosines are not accessible to the enzyme and thus are probably located on the cytosolic side of the membrane. Carbohydrates are present only on the exterior surface of plasma membranes. The presence of a carbohydrate unit at position 302 thus shows that this portion of the protein is on the exterior surface. The simplest interpretation of the above results is that the protein makes 3 passes through the membrane with its COOH-terminal region (residues 594-780) and an internal segment (residues 176-335) present on the exterior surface. A diagram of
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This note was uploaded on 05/30/2010 for the course BIS BIS 104 taught by Professor Privalsky during the Spring '09 term at UC Davis.

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Key+Assignment+1 - BIS 104, Section 1 Spring Quarter, 2009...

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