Lecture7 - Lecture 7 4/20/10 Background reading: MBOC 5 th...

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Unformatted text preview: Lecture 7 4/20/10 Background reading: MBOC 5 th Edition: Chapter 12: Pages 736 – 739 Chapter 13: Pages 749 – 753; 771-775; 779-787 4 th Edition: Chapter 12: pages 702 – 704 Chapter 13: Pages 731-737; 739-745 utline: • Co- and posttranslational modification of proteins in the ER • Secretory system • Pulse chase experiments • Green fluorescent protein (GFP) fusion proteins • Golgi apparatus • Processing of N-linked carbohydrate unit • Retention of resident proteins of ER • Lysosomes • Targeting of proteins to lysosomes • Inclusion cell disease (I-cell disease) • Co- and post-translational modifications of proteins in ER d) N-Glycosylation Consensus sequence: N X S/T S S C C NXS Asn-X-Ser Expanded view of the carbohydrate unit transferred onto the protein in the ER = N-acetylglucosamine (GlNAc) = Mannose = Glucose While in the ER, all three glucoses are trimmed off of the carbohydrate unit in a controlled process that assures that the protein is correctly folded before it can move onward in the secretory system. • Secretory System Route traveled by all proteins destined for following locations: Endoplasmic reticulum Golgi apparatus...
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This note was uploaded on 05/30/2010 for the course BIS BIS 104 taught by Professor Privalsky during the Spring '09 term at UC Davis.

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Lecture7 - Lecture 7 4/20/10 Background reading: MBOC 5 th...

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