Lecture10 - Lecture 10 5/6/10 Background reading: MBOC 5th...

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Lecture 10 5/6/10 Background reading: MBOC 5th edition: Chapter 16: pages 965 - 991 MBOC 4th edition: Chapter 16: pages 907 - 929 Outline: Microtubules Microtubule dynamics Drug sensitivity of microtubules Regulation of microtubules Organelle movement Motor proteins Actin Treadmilling Regulation of polymerization Motility and cell shape
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Microtubules Long, stiff polymers extend throughout the cytosol and govern location of organelles. Hollow tubes formed from tubulin. These dimers polymerize to form a protofilament. Protofilament
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Thirteen of these protofilaments form a hollow cylinder, which is the microtubule. The microtubules have polarity. All of the protofilaments are aligned with the same polarity with an a or b end facing each end of the microtubule. minus end plus end The microtubules are long and straight and usually have one end (- end) attached to a single Microtubule organizing center (MTOC) , called a Centrosome. The centrosome usually lies next to the nucleus and serves as the nucleation center of the microtubule. The other end (+ end) of the microtubule is capable of rapid growth.
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Microtubule dynamics: GTP binding to the β subunit is important for the dynamics of microtubule assembly and disassembly. Microtubules are dynamic structure that continuously assemble and disassemble. GTP γ S is GTP analogue GTP binding but not hydrolysis is required for polymerization of tubulin. Guanine O -- P =O O O - P =O O O - P=S O -
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Dynamic Instability: Steady growth or rapid disassembly.
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Drug sensitivity of Microtubules: Microtubules are sensitive to a variety of drugs. Examples:
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Lecture10 - Lecture 10 5/6/10 Background reading: MBOC 5th...

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