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Unformatted text preview: 3. Mix with dye and let it sit for about five minutes. 4. Measure the absorbance of each of the solutions. 5. Create a graph of the absorbance of all the solutions. 6. Then calculate the concentrations of the unknown sample solution with the best fit line. Results Concentration (M) Absorbance (nm) Blank, 0 .25 .361 .5 .550 1 .830 1.25 .985 Sample y = 0.7357x + .1038 .714 = 0.7357x + .1038 x = 0.829 .714 (See Graph) Conclusion Using the best fit line we were able to find the concentration of the protein in the unknown sample. The graph relates the absorbance and concentrations. We learned how to effectively use the Bradford method....
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- Spring '10