12 - Monday October 12th 2009 Biochemistry 405 Lecture#6...

Info icon This preview shows pages 1–13. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Monday, October 12 th, 2009 Biochemistry 405 Lecture #6 Kane Hall 130 10:30- 11:20 am Lecturer: Wim Hol Slide Set #1
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
2 Outline 1. Quarternary structure and Symmetry. - Point Group Symmetry - Helical Symmetry - Partial Symmetry - Non-symmetrical 2. Forces stabilizing protein structures. 3. Protein folding - Levinthal Paradox. - Protein folding - Protein Disulfide Isomerase - Chaperones 4. Folding Diseases Quaternary Structure & Protein Folding
Image of page 2
3 The arrangement of multiple protein chains into a larger assembly is called the quaternary structure of that assembly. In living cells, many multi-protein assemblies occur. The advantages if bringing multiple proteins together are several, including: - multiple functions can be brought in close proximity , as in multi-enzyme complexes and in bacterial toxins; - cages of sufficient size can be created, as in chaperones; - long fibers can be obtained, as in microfilaments . Multi-protein assemblies can: - have symmetry, or partial symmetry, or no symmetry at all; - consist of multiple copies of a single, of a few, or of numerous different proteins. Quaternary Structure
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
4 Cyclic Point groups Dihedral Point Group Symmetry Cubic Point group Symmetry 7 7 The Three Types of Point Group Symmetry Arnthor Aevarsson Point group symmetry: all symmetry axes intersect in one point, the center of the particle
Image of page 4
5 Haemagglutinin From the surface of the influenza virus A Trimer with Cyclic C3 Point Group Symmetry Schematic
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
6 A Heptamer with Cyclic C 7 Point Group Symmetry Shekhar Mande GroES The cap of a protein folding machine Side view Top view
Image of page 6
7 Cyclic Point groups Dihedral Point Group Symmetry Cubic Point group Symmetry 7 7 The Three Types of Point Group Symmetry Point group symmetry: all symmetry axes intersect in one point, the center of the particle
Image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
8 Schematic GroEL Dihedral D7 Point Group Symmetry GroEL: The body of a protein folding machine, a 14-mer
Image of page 8
9 Cyclic Point groups Dihedral Point Group Symmetry Cubic Point group Symmetry 7 7 The Three Types of Point Group Symmetry
Image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
10 Quaternary Structure The Three Types of Cubic symmetry A game of intersecting 2-folds, 3-folds, 4-folds and 5-folds Only 2-folds and 3-folds 2-folds, 3-folds and 4-folds 2-folds, 3-folds and 5-folds Tetrahedral Symmetry Octahedral Symmetry Icosahedral Symmetry
Image of page 10
11 24-mer with octahedral symmetry of eight E2 Catalytic Domains trimers View along 4-fold axis Only the 4 trimers in the front are visible. Example of Octahedral Symmetry: a hollow truncated cube Andrea Mattevi The Core of the Pyruvate Dehydrogenase Multi-Enzyme Complex 3-mer viewed along the 3-fold Three E2 Catalytic Domains (Each color is one domain) (Each sphere is one amino acid residue) X 8 =
Image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
12 Several viruses have icosahedral symmetry RHINO VIRUS (human common cold virus) VP1, VP2 and VP3 subunits are quite similar in fold.
Image of page 12
Image of page 13
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern