mol4 - BIMM100 Lecture 4! On the way to more DNA!...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
BIMM100 Lecture 4 On the way to more DNA Waitlist has cleared. Check WebCT for problem sets replication & repair processes
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Chapter 4 Finished - nucleic acids transcription translation Continuing - replication damage and repair viruses Next week - Chapter 5 Molecular Genetic Techniques (review 165-176) Remember Exptl Fig 4-29 Chapter end: Terms, Concepts, Data** all except 8 NOT COVERING HOMOLOGOUS RECOMBINATION(pp 150-153)
Background image of page 2
Lecture 4 – Key Molecular Processes DNA Replication The process The players Coordination and bi-directional replication Finishing DNA Mistakes, Damage and Repair Polymerase – errors and proofreading A segue: Why we care A gallery of damage and repair deamination base excision mismatch repair UV damage and nucleotide excision repair global vs. transcription coupled NHEJ – last chance Viruses, briefy
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Key Molecular Processes III E. coli: 1000 bp/sec/fork 4,639,221 bp genome Human: 100 bp/sec/fork 3x10 9 bp genome Replication ~ 8 hours How can this be???
Background image of page 4
ʻ problems ʼ arising from DNA structure and polymerase unwinding priming polarity (and directionality) ends of linear molecules speciFc enzymes and replicative processes ʻ solve ʼ these problems
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
DNA replication beginning the process the site: replication ʻ origin ʼ sequences are A-T rich size, sequence variable helicases unwind DNA at the origin primase (an RNA polymerase) -- short, complementary RNA primers on both strands then -- elongation by a DNA polymerase site of action: replication fork (growing fork)
Background image of page 6
DNA replication solving the polarity problem Also --replication occurs bidirectionally from each origin net: ʻ leading ʼ strand: continuous synthesis ʻ lagging ʼ strand is discontinuous
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Pol α -- extends primers Pol δ -- elongating polymerase Other key players: Rfc, PCNA
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 35

mol4 - BIMM100 Lecture 4! On the way to more DNA!...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online