mol8 - BIMM100 week 4 (lecture 8) Tricks for inactivating...

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BIMM100 week 4 (lecture 8) ‘Tricks for inactivating genes’ Stephen Gilmore Graduate Student in Dr. Pillus’ Lab Email- sgilmore@ucsd.edu Midterm Next Thurs(4/29) Peter 108/Center 216 BIG REVIEW Tues 7-9 Pep Can 109 Help Rooms Pac Hall 3501/2 Mon 2-4 Wed 3-5 Practice midterm posted TODAY For Tues: Expression and midterm review
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Mon Tues Wed Thur Fri Sat Sun 22 23 24 25 26 27 28 29 8 am Sections Lecture 8 11 am 12 pm 2 pm 1 pm Lecture 9 1 pm 7-9 p.m. Review Pepper C 109 9:30 OH Office Hours (TA) Office Hours (Prof Pillus) 11 am OH Special Events 12:20 AG 4 SG 8-10 AY 2 CE Practice MidTerm Posted @ WebCT 2 SF 1 RC 8 am 3-5 Q & A Pac Hall 3501 & 3502 Exam Peter108 & Center216 Bird’s Eye View Heading to BIMM 100 MidTerm 2-4 Q & A Pac Hall 3501 & 3502 7 pm 11 Am 12pm PLAY GRADE +
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Inactivating Genes THE BIG QUESTIONS - Why knockout a gene? Is a gene essential? What is the function of a gene within the cell? What role does a gene play in the development and maintenance of a whole organism?
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Approaches to Gene Disruption A particular gene’s expression can be decreased or eliminated by … Genomic disruption of the gene of interest • Generating mutant yeast • Generating mutant mice •Tissue specific knockouts Post-transcriptional gene disruption • Using RNAi to block gene expression • Progress with Rat knockouts
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Yeast! Also a wonderful model organism for genetic manipulation and study Very high efficiency of homologous recombination Ability to propagate stably as both haploid and diploid cells Creation of a gene knockout collection and GFP- tagged gene collection. …in addition to these fine products…
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A quick reminder: meiosis
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Gene Inactivation in Yeast Gene inactivation in yeast ( S. cerevisiae ) 1. Make a disruption construct 2. Introduce it into yeast cells 3. Select for cells that incorporated the disruption construct 4. Study cells with the deleted gene of interest.
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mol8 - BIMM100 week 4 (lecture 8) Tricks for inactivating...

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