mol18 - BIMM100-Lecture 17 Final words about...

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BIMM100-Lecture 17 Check WebCT for Final Info Details Preview: Review Session Sun afternoon (6/6) 4-6pm Center 101 Help Rooms ( still trying to get rooms ) Friday afternoon (4/6) Monday afternoon (7/6) Post-transcriptional Regulation Chapter 8 Final words about termination then Exams available for pickup 3100E Pacific Hall MCB 7-51 Anti-termination in Pol II transcription of HIV HIV tat encodes a small protein, binds to RNA TAR sequences TAR has stem-loop structure, bound by both Tat and Cyclin T Cyclin T interacts with Cdk9, phosphorylates CTD, promoting elongation Without Tat, transcription stops after ~ 50 nucleotides Net :Tat protein is an anti-termination factor (target for therapeutic intervention) Spt4,5 and NELF ordinarily facilitate constitutive elongation Stimulation of Transcription by HIV-1 Tat Protein Antitermination (Flint 8.14) Multiple copies of TAR are transcribed 1 in 10 transcripts made is a longer transcript that encodes the protein Tat Stimulation of Transcription by HIV-1 Tat Protein Antitermination (Flint 8.14) Tak Tat induces phosphorylation of RNA Pol II (at the CTD) further stabilizing RNA Pol II and increasing transcription levels
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Post-transcriptional gene control -- big picture MCB 8-1 Overview Capping RNA binding proteins Splicing Spliceosomes Self-splicing Coordinating processes mRNA processing: the overview nucleus cytoplasm MCB 8-2 Life cycle of an mRNA - action! capping the 5 ʼ end of nascent* transcripts * in the act of being born; just forming after RNA 25-30 nts dimeric capping enzyme assoc ʼ d with CTD-p, steps 1,2 phosphohydrolase guanylyltransferase guanine- 7-methyltransferase 2 ʼ - O -methyltransferase (capping enzyme does not associate with pol I or III,
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This note was uploaded on 06/03/2010 for the course BIMM Pillus taught by Professor Molecularbiology during the Spring '10 term at UCSD.

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mol18 - BIMM100-Lecture 17 Final words about...

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