2_1_Picornavirus_I_poliovirus

2_1_Picornavirus_I_poliovirus - Picornaviridae (poliovirus)...

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Unformatted text preview: Picornaviridae (poliovirus) 5' fl—P‘l—M—PZ ‘ P4 VP2 VP3 VP é! nnfllu Illilllll aa VPg T 3. IIIl Aphthavirus 2A Figure 2: Genome structure and gene organization of picomavlruses. The filled circle at the 5' end is the genome- linked protein Vl’g (also referred to as the 38 gene product), followed by the 5' non-tramlated region (5‘ NTR; line). Letters (C) above the 5’ NTR depict poly (C) tracks that are presentin some viruses. The 1A gene products are myristylaled at the amino terminal glycine, although the situation for hepalnviruses is not known, The open boxes depict the long ORF encoding the polyprote‘m that is followed by the 3' non-translated region (line) and a poly (A) track (angled line). The eventual cleavage products of the polyprotein are indicated by vertical lines in the boxes, the nomenclature of the polypeptides follows an L:4:3:4 scheme corresponding to the genes (numbers) encoded in the L, P1, P2, P3 regions (Rueckert and Wimmer, 1984). h——— mono—«fl non-capsid——-——-—-d p—-—p/————~—P2——-———P3—-—H IhIViaf/bn of trans/arm MO 5372 5987 74M 7370 C. E. Samuel MCDB 134 5— 3' 5 6| The replication of single—stranded RNA. thure' 1. Schematic RepresematiOn of the Genetic Map of Poliovirus The genome RNA is shown as a solid line to- gether with the precise map positions at the gene products. in nucleotide numbers of type 1 (Mahoney). Wavy lines indicate the polypep- 3 l ‘ VPg-PU : i 3 : g 3 5; g g ‘33:) tides. their calculated molecular weights (kib- 5’ 5 'z i ‘ Swim/MW” i ii 1 ‘v ‘ daltons) indicated in brackets. Solid triangles: -'r r' " i l l -, 3" g '\ termination of O—Gcleavagosites.0penlrianglas:Y-G cleav- 1 " Pr}er " I : Flaw ' \‘ Aim; : "W’s/WU” 399 sites. Open diamond: N-S cleavage site i y t ; v ‘. l 1" \ ‘ (modified after Kltamura at AL. l981). JV, I?) 'VPJrn} Via/3032‘: EEC/u; :gTro/‘v‘ “ WIN/Paul 325'?ch VPg/glwmww proteases 2A, 3C M 1 . ': polymerase 3D «same: mil/sup ‘ ! ii 1 'Im, l G." u‘c , 20-6 c 1|“! 5 o u ‘1: W o' “'1 0 I o V i 1 o POL10MYELITIS 'r m “TERIOR 6‘" bun-Act tug ‘ menu-mmmuugccmonmm (A COMM UNICABLE DISEASE) Keep Out of this House Any pun-a nang um um vim-mt authority In um. u firemen By Order of BOARD OF HEALTH HEALTH OFFlCER ‘— Figure 20.9 Poliovirus in the early 20th century. ‘ Board of Health quarantine notice, San \, Francisco, Calif, circa 1910. 1 ‘ Ltu , | 1 Wu >Am Pm'lin ' Vul- Flu—"Iv - ll. {5}]11- In‘ ValvGIn-COO' O to so to 1'!) no Fig. 3 Structure of the 5’-lerminus of poliovirus RNA. The oligonucleoti‘de (n =85) is an RNasc Ill cleavage fragment of virion RNA . The viral protein VPg is linked via an O‘—(5'- uridylymyrosine (T yr-pUp) bond to the RNA 1 t TABLE 1. Viral characteristics: the genera of Picornaviridae' HAV Enterovirus Rhinovirus Cardiovirus Aphthovirus Characteristic (HM175/wt) (PV 1) (RV 14) (EMCV) (FMDV A12) In vivo Serotypes 1 (213 >70 >130 2‘ (26 strains) 7 (253 strains”) subtypes) Primary hosts Humans, other Humans. other Humans. other Mice, other Cloven-tooted. primates mammals mammals mammals other mammals Host range and tissue Narrow Narrow to wide Narrow Wide Wide tropism Primary habitat Liver Gut Upper Central Generalized respiratory nervous tract system, heart Sensitivityd Acid (pH 3)‘ Stable Stable Labile Stable Labile Heat (60°C, 60 min) Stable Labile Labile Labile Labile ‘ Guanidine Resistant Sensitive Sensitive Resistant Resistant Disoxaril ' Resistant Sensitive Sensitive Resistant Resistant Biophysical" Buoyant density (g/cm“ in 1.32—1.34 1.34 1.39—1.42 1.34 1 .43-1.45 CsCI) Sedimentation coefficient (S) 156—160 156—160 149 156 142—146 Virion proteins (M, x 10‘3)" VP1 33.2 33.5 32.4 31.7 23.3 VP2 24.8 30.0 28.5 29.0 24.7 VP3 27.8 I 26.4 26.2 25.1 24.3 VP4 525‘ 7.4 7.2 7.2 8.5 VPg 2.4 2.3 2.4 2.2 2.6—2.7’ Genomek Length (kb) 7.43 7.44 7.21 7.84 3.4 as (G + C) 38 47 40 50 43 Poly(C)' - — - + + Poiy(C+T)’ + - — - - Similarity“ HAVs Enteros., Rhinos. Cardios." Aphthos." Rhinos." Entercs." J ' After tables in refs. 77, 156, and 437. Certain data (guanidine sensitivity, M, of proteins. genome length. % [G + ~‘ Cl) cited for particular strains or subtypes: HAV strain HM175/wild type (HM175/wt). poliovirus type 1 Mahoney (PV 1). rhinovirus type 14 (RV 14), encephalomyocarditis virus (EMCV), and toot-and-mouth disease virus type A12 (54.68226.323.325.342357,365.416). For sources of other data, see text of this chapter and Chapter 20. Infection E ingested virus \ Regional nerve ganglia ' ‘ ' " based on a synthesis of data ob— FI . 55-9. A model for the pathogenesus oi pollomyelitis I I taigned in man and chimpanzees. [Modified from Sabin, A. B. Science 123.1151 (1956) and Rnrllan n .Qm'enma 199'10511955l.l ‘ - t " ‘1'": Alimentary I: Qropharyngeal mucosa ——u—Virus in throat ——-- Intestinal mucosa g g A phase I a D \ o I \ / g u \ / _ a \ / ' h .5 : Tonsils \ Virus in feces / Peyer s polc es § E 333 Lymphatic // _ I: I / ,6 I I phase a I Mesenteric lymph nodes i; a u a '0 — _ . H O a E a I I: a ‘ g; 0 8 Mag 5 3 2 u 5 .a I. g §—> "’ '8 G l' ' c 3azé In L) Viremic I I I 2: '5 ag g (responsnble for persisting a E U phase I I g > vrremia) e E O — G 3 2 8 2 ° = «5% g ' le uone|ndod ooo'ool .Iad o a‘ u Central nervous s stem Other suscepth 2 u I: y exfraneural tissues 533” P9310“)! 2 : Neurological a: 3 h ._ .a 53 p use II- F‘ {— m ...
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2_1_Picornavirus_I_poliovirus - Picornaviridae (poliovirus)...

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