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3_1_Rhabdovirus

3_1_Rhabdovirus - C E Samuel Ehgbdpyrerdae(veSIeular...

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Unformatted text preview: . . . . . . . C. E. Samuel Ehgbdpyrerdae (veSIeular stomatltls v1rus) MCDB 134 vsv GENOME e as RNA I 2 2 2 2 “$62!! 10kt: Bkh Bk!) 7 kb Shh 5H) 4": 3k!) 2“: lkb 1H5 Zkh 3th 4RD 5RD Bkh “lb Bib 9 llh 10“: HIGH! FIG. 5. VSV genome. The nucleotide sequence analysis of the L gene. together with the previously reported sequences of the leader region (I). the N. NS. .\I. and (i. genes. the four two-nucleotide intereistronic regions. and the trailer region it). completes the sequence of the VSV geltome.'The coding regions of each gene are marked by closed boxes. The sizes ofthe messages without polytAl tails and the terminal untranslated regions of each message are indicated above and below. respectively. The genome of VSV is 11.162 nucleotides long. of which the L gene alone comprises 57.4%. ()f the nucleotides in the genome. 99.4%“: transcribedand 93.9% are translated. Genomic RNA(-) Leader mRNA MRNA mRNA mRNA mRNA ‘ x ' I N +‘ strand i ll \ l mRNA synthesis 1 — .. ‘ , ' i '1 The switch from mRNA synthesis to genomic RNA rephcauon is . ' regulated by the viral nucleompsid (N) protein. When enn- NeuNAn NeuNAr.‘ NeuNAc l l l 0 CH] Man Man Gd Gd Gd N r .J H "1 1 1 1 \ . an an an (1th GlcNAL- Glob/Ac H g I > we. 1 \ / 1 \ / Z“ N N . . 5- E h) g . Man Man Man Man cm-o-w-o-p-o-mo—Eu, ' \ / \ / a- s,- 1— "a," "a," i r. GliNAc GlcNA: , l ’1 GlclNAa: GlcNAc ‘ -Am-X-S¢rfl'hr- -Ain-X-S¢rfl'hr- ‘ Figure 1. Structure of 5' Cap even on mature utvconmrains, 3. 59113 ' 10.110 29:110 41.146 10:54 13:50 a t . | I I l I l t t l l FIG. 4. Mannose-rich (left) and complex (right) N-linked o-P—o--- oligosaccharides. The mannose-rich oligosaccharides are >. . composed of N-acetyl»glucosamine (GlcNAc) and mannose 0- (Man). Carbohydrate processing enzymes in the Golgi remove the outer mannoses and add fucose, N-acetyl glucosamlne, galactose, and terminal N-acetyl neuraminic acid (NeuNAc, sialic acid). Not all oligosaccharide chains are identically processed. The structure shown here is only one example. Moreover, some chains remain as mannose-rich structures, centrations of N rotein are low. mRNA synthesis is tavored (top). When the gt protein concentration is higher, it binds to nascent (+) strands and allows the RNA polymerase to read through the intergenic junctions (is) at which polyadenyia- Lion and termination take place during niRNA synthesis. ...
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