Week3answers_2009

Week3answers_2009 - Order of transcription of genes of...

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Order of transcription of genes of vesicular stomatitis virus Andrew Ball and Carol White PNAS, 1976 MCDB 134 week 3 discussion Section scores: Answers to questions on group paper. Understanding of paper. Preparation/contribution. Teamwork. * Midterm 1 WILL have some questions related to section information.
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Make up assignment for those who missed section (you can only do one make up assignment this quarter) This make up paper should be at least one page. Summarize the important information from this paper including the methods used, the results of the experiments and conclusions reached by the authors. Be sure to emphasize the goal of the research and the extent to which the goal was reached.
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Important Background Vesicular Stomatitis Virus (VSV) Rhabdovirus Negative ssRNA virus Transcribes positive sense RNA (mRNA) Makes five proteins: 1. N – nucleocapsid protein (packages genome) 2. NS/P – nonstructural polymerase component 3. M –matrix (condensation of nucleocapsid, budding of virus) 4. G – the glycoprotein (attachment of the virus to the cell) 5. L – the RNA dependent RNA polymerase (RNA transcriptase)
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VSV Structure and Genome Do the viral transcripts (mRNAs) arise from: I. Initiation from 5 separate promoters? II. A single promoter performing one long read through followed by processing of the transcript?
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Background continued…. UV irradiation produces thymine dimers in DNA. These dimers inhibit transcription past the damaged site. This is known as single-hit inhibition of virus replication. VSV genome Thymine dimer = mRNA no protein produced 1. What is the direction of a) replication of a genome? b) transcription of a genome? c) translation of a genome? d) amino acid addition to a protein? What is wrong with this slide?
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Remember, all polymerases synthesize their products in the 5’ to 3’ direction (5’ to 3’ in relation to the product). This means that they must progress from 3’ to 5’ in relation to the template. Also, ribosomes travel 5’ to 3’ along a mRNA. What is the direction of: a) replication of a genome? 3’ to 5’ in relation to the template, 5’ to 3’ in relation to the product b) transcription of a genome? 3’ to 5’ in relation to the template, 5’ to 3’ in relation to the product
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This note was uploaded on 06/08/2010 for the course MCDB 134 taught by Professor Samuels during the Spring '10 term at UCSB.

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Week3answers_2009 - Order of transcription of genes of...

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