Mapping_of_the_influenza_virus_HA_and_NA_genes - Mapping of...

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Neuraminidase Genes Mapping of the Influenza Virus Genome: Identification of the Hemagglutinin and the Peter Palese, and Jerome L. Schulman doi:10.1073/pnas.73.6.2142 1976;73;2142-2146 PNAS This information is current as of March 2007. This article has been cited by other articles: E-mail Alerts . click here top right corner of the article or Receive free email alerts when new articles cite this article - sign up in the box at the To reproduce this article in part (figures, tables) or in entirety, see: Reprints To order reprints, see: Notes:
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Proc. Natl. Acad. Sci. USA Vol. 73, No. 6, pp. 2142-2146, June 1976 Microbiology Mapping of the influenza virus genome: Identification of the hemagglutinin and the neuraminidase genes (RNA/recombinant viruses/polyacrylamide gel electrophoresis/genetics) PETER PALESE AND JEROME L. SCHULMAN Department of Microbiology, Mount Sinai School of Medicine of the City University of New York, 100 St. and 5th Avenue, New York, N.Y. 10029 Communicated by Robert M. Chanock, March 16,1976 ABSTRACT Polyacrylamide gel electrophoresis of the RNA of influenza A/PR/8/34 (HONI) and A/Hong Kong/8/68 (H3N2) viruses and recombinant viruses derived from them revealed that each contains eight RNA segments, the fourth of which codes for hemagglutinin. (The largest RNA of the segmented genome is counted as band 1.) The neuraminidase gene was identified as the sixth segment in the RNA pattern of influenza A/PR8 virus and as the fifth segment of A/Hong Kong virus. ITe molecular weights of the RNAs for the hemagglutinin and the neuraminidase genes lie in the range of 600,000-700,000. Analysis of RNA of influenza A viruses has revealed that the segmented genome contains at least seven distinct RNA pieces (review, ref. 1; refs. 2-6). These observations are in accord with the results of genetic experiments involving studies of recom- binational frequencies among different strains of virus and characterization of temperature-sensitive mutants, which have indicated that transcripts of each of the virion RNA segments probably function as monocistronic messages coding for a single virus polypeptide (review, ref. 7). The sizes of the different virion RNA segments range from a molecular weight of approximately 1 X 106 to 2 to 4 X 105 (5, 6). Presumably, complementary RNA (cRNA) transcripts of each are capable of coding for corresponding proteins with molecular weights ranging from 1 X 105 to approximately 2 X 104, a range which corresponds with that of isolated virus pro- teins (review, ref. 8; refs. 6 and 9). Although correlations in size have been used as a basis for speculation, it has not been possible until now to identify specifically which RNA segment is the gene for any specific virus protein.
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This note was uploaded on 06/08/2010 for the course MCDB 134 taught by Professor Samuels during the Spring '10 term at UCSB.

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Mapping_of_the_influenza_virus_HA_and_NA_genes - Mapping of...

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