Week_4_2009_Influenza_Palese_answers

Week_4_2009_Influenza_Palese_answers - Make up assignment...

Info iconThis preview shows pages 1–11. Sign up to view the full content.

View Full Document Right Arrow Icon
Make up assignment Write a one to two page summary of this week’s paper. Include the information highlighted in this presentation along with conclusions made by the authors. Be sure to explain how the experiments were done and the significance of the results.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Mapping of the influenza virus genome: Identification of the hemagglutinin and neuraminidase genes Peter Palese and Jerome Schulman PNAS, 1976 MCDB 134 week 4 discussion
Background image of page 2
Background Influenza Family orthomyxovirus Segmented genome: 8 segments (-) ssRNA Enveloped virion, encodes two surface proteins: hemagglutinin (HA) and neuraminidase (NA). Goal of the Paper To determine what influenza virus genome segments contain the hemagglutinin (HA) and neuraminidase (NA) genes.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Method Background Influenza strains are characterized by their serotype . This is determined by the antibodies that recognize them. The two primary surface antigens are HA and NA . Antibodies that specifically recognize one HA or NA type do not recognize another. The serotypes are given numbers (e.g. H0 or H3, N1 or N2). Two strains used for the study: PR8 (H0N1) and HK (H3N2).
Background image of page 4
Re- assortment
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Very few genome segments come from the UV treated virus.
Background image of page 6
Why is UV treatment needed? A B A + B 1. Coinfect virus A and B. 2. Select for a gene product from virus A. 3. Gel electrophoresis with progeny genome progeny virus A+B has more than one gene from virus A. Treat virus A with UV to minimize the number of gene segments that the progeny viruses contain. Parents Progeny
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
H- N- H3N2 ( HK ) - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - H3 N1 eggs - = genome segments ? genome segment Select what is wanted by getting rid of what is not wanted using antibodies. - - - - - - - - H0N1 ( PR8 )
Background image of page 8
2. a) What is the goal of the Palese and Schulman paper? b) Briefly, what method did the authors use to reach their conclusions? c) What characteristics of influenza viruses allowed the authors to use this strategy?
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2. a)What is the goal of the Palese and Schulman paper? See the next slide.
Background image of page 10
Image of page 11
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 06/08/2010 for the course MCDB 134 taught by Professor Samuels during the Spring '10 term at UCSB.

Page1 / 21

Week_4_2009_Influenza_Palese_answers - Make up assignment...

This preview shows document pages 1 - 11. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online