6_2_Papovavirus_SV40

6_2_Papovavirus_SV40 - C.E.Samud Papovaviridae(SV40 MCDB...

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Unformatted text preview: C.E.Samud Papovaviridae (SV40) MCDB 134 emaNAs and the 29§§ILQQ§QLIQHQQ§J§LQEM§LM early region extends counterclockwise and the late re- p. The regions mm viral proteins are shaded. Also shown are U C (U C .9 ‘5 .2 a 9 ‘6 E Z? ‘6 0 .C F 0' v > w E 9 1' ,: E § a 5 . _: g '8 ~' 8 ‘m _> 3 H5 " éé __8 {g g i" 2% 533 m g '5; § SN 8 0 32 .m a g E’— g? 2 ~ g N ri‘ 3:» 0 U0 ~I M n C‘— 0) l1"; “‘1’ 'c v 05 : E's “’ comgf‘ m—‘_fl)m OCCm 1:2“: gust! Egga 'er Sgggv 9332’7‘ oax'asl 2Q§gaz El‘owlfl oa§§n n‘CXU-C wave: Qfigwo‘ LLz'EEN < E CHAPTER29 320 240 160 80 0/5243 51 63 bp ; l Late mRNAs Early mRNAs ;' Jl—UJ—lllLl—l__l_l___J WE- WEE- l Egggwrfer ED g ca Early Promoter l A 3329332? Pawnee llllllllllfll%i% or no aux-1 Ori-Core Domains :3 ! A A Ongm of Bidirectional Replicatlon 21> FlG. 3. The regulatory regions of SV40 (A) and polyoma (B). l6$ Hgm 2.2 Diagrammatic representation of the different confi t’ DNA molecules of polyoma virus and their 3 gum Ions 0f the edimcntation coefficients. no 6 Model of the preinitiation complex based on that of Buratowski et 3 al.” The first critical step in the moleatlon of the prelnltlation compiex is i thought to be the binding of the TFIID complex to the TATA box element 5 through one of its subunits. the TATA-box-binding protein (TSP). The some- ; what unstable binding 01 the TBP—TFIID complex to the TATA box may be ‘ stallized by the binding of TFIlA (A). THE (B) is Wt to bind next. RNA l Polllintnepmofmfilficantnenmmbindtomis 1 ‘ DNAMW. THE (E) can then enter them oompiex. Enhmeer-binding proteins /\ Initiation complex L. , -s h.,___.._-._m-... . w"... r;- m. m an: o, Figure 8J8 Mechanisms of enhancer action. (A) The DNA looping model postulates that proteins bound to a distant en- hancer, here shown upstream of a gene, interact directly with oomponents of the transcription initiation complex, with the intervening DNA looped out. These interactions of proteins bound to a distal enhancer are analogous to those that can take place when the enhancer is located close to the pro— moter. Such interactions might stabilize the initiation com~ i pleit and thus stimulate transcription. TATA 5. 3. Relative transcription Figure 8.” Organization of the simian virus 40 enhancer. The positions of the 72-bp 1 repeat region of the simian virus 40 genome containing the enhancer elements relative to l the early promoter are shown at the top. Shown to scale below are functional DNA se- quence units. and the proteins that bind to them, of the early promoter—distal 72-bp repeat and its 5’-flanking sequence, which forms part of enhancer element B. All the protein- binding sites shown between the expansion lines are repeated in the promoter-proximal 72-bp repeat. Thus, the complete enhancer contains one copy of the enhancer element Bl SV40 LARGE T ANTIGEN 708 600 400 200 BINDING 5 5 < Ill 0: < 2 .1 w I d z o l—.....___.__| Transformation l—————-—-——-—' Immortfllzaflon l——--—-—l Induction Host DNA I—-————_—_l DNA POLa Binding Transcriptional Activation ...
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