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7_2_Retroviruses_I.2008

7_2_Retroviruses_I.2008 - Retroviridae Rous Sarcoma...

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Unformatted text preview: Retroviridae Rous Sarcoma Virus (RSV) Human Immunodeficiency Virus (HIV) ”' ALV DD————————.‘——‘_D]] pol env HTLV Eln—————————————E1D E} C: gag 1:] W E: pro env H" tax 4:] a ------- III rex HIV Em——————————IZIII E: E] [:1 gag - Vlf a env ::::I We -------- IIEZI tat pro pol fl- _______ Dnef El rev vpu 0 2 4 6 8 10 kb I I I I I l FIG. 5. Coding regions of retrovirus genomes. Open reading frames in the genes of representative mem- bers of each retrovirus group are shown relative to the proviral structure. Sites of translation initiation are shown (small arrows). Note the different orientation of pro and pol relative to aaa. and the overlap of gag and envin most viruses. Sequences from refs. 756.481 ,678,513. FIG. 4. Sequence features of retrovirus genomes. t ® Rous Sarcoma Virus CAP +4538“) <—(x10)—> PB") U3 RAA2OO P2216“ 1215 a @4p324 gpgs gpfii ‘ ppGO 3' r r p Ga] 5053:69er ;1/9_______EL1§_Q______“L§7 ppeo Gas- Poi Prawn»; V. C. E. Samuel MCDB 134 ASLV RT MLV HIV-I [3(95 kDa) a (63 kDa) IN RT (80 kDa) IN p66 RT pSI RNase H IN Cell DNA CAOH i Proviral DNA AATG GT:AA HoAc I l I GGGC CCCGgg Chapter 25 / Retroviruses l Integrase Cell DNA Polymerase I —-——-——-G 79Te __ °” ”5 ‘m CCCGG ACW CAGGCCC 7,, Host repair enzymes R U5 CAGGCCC Proviral DNA GTCCGGG Cell DNA FIGURE 25.5 Integration of proviral DNA into host cell DNA. V ital integrase cleaves 2 nucleotides from the 3' ends of the viral DNA, then joins these 3’ ends to host DNA in a concerted cleavage-ligation reaction at sites 4—6 m apart on the host DNA (dashed arrows). Host cell enzymes repair the resulting gapped structure. Proviral DNA is orange, host DNA is black. (5) Figure 7.6 Domain and subunit rela- tionships of RTs from different retro- viruses. The RT of ASLV includes sequences corresponding to integrase in its largest subunit, B. Both subunits of this enzyme include RNase H sequences. MLV RT functions as a homodimer; both subunits contain the RNase H domain, but neither includes 1N sequences. Like that of ASLV, the HIV-1 RT is a het- erodimer. However, in this case only one subunit includes the RNase H domain, and neuher indudes IN sequences Adapted from R. A. Katz and A. M. Skalka, Annu. Rev. Biochem. 63:133—173, 1994,udfl1pernusnon. ...
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