Week_7_2009_Baltimore_ANSWERS

Week_7_2009_Baltimore_ANSWERS - Each discussion group no...

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Unformatted text preview: Each discussion group no more than 4 people Quiz #1: I still have some unclaimed ones Midterm II is coming up within one week, next Thursday office hour from 1:00PM 3:00PM, same room. There are two practice problems at the end of this file, one for Baltimore paper, the other one for Berk & Sharp paper again. Week 7 Make-Up Assignment Write a 1-2 page summary of the Baltimore paper. In your summary, explain the observations that support the hypothesis of the existence of RNA- dependent DNA polymerase, then describe the assay Baltimore used in this study and what kind of data can be obtained with this assay. Finally, write a concise but complete explanation of the data and state the conclusions made from the data. Viral RNA-dependent DNA polymerase in virions of RNA tumor viruses David Baltimore Nature 226: 1209-1211 (1970) MCDB 134: Week 7 Discussion li@lifesci.ucsb.edu About Retroviridae A diverse family of viruses found in all vertebrates. Some family members: HIV, RSV, MLV. HIV is a very important pathogen to human Unique from other viruses in that they have a RNA genome that is reverse transcribed into a DNA form that is integrated into the host chromosomal DNA. The enzyme responsible for reverse transcription is tremendously useful in the laboratory. Reverse transcription and formation of the provirus +ss RNase H, RT RT Reverse transcriptase (RT) ssRNA DNA RNA Newly formed provirus DNA DNA Reverse transcription is now known catalyzed by reverse transcriptase, the DNA polymerase uses RNA as template Reverse transcription and formation of the provirus +ss RNase H, RT RT Reverse transcriptase (RT) ssRNA DNA RNA Newly formed provirus DNA DNA Reverse transcription is now known catalyzed by reverse transcriptase, the DNA polymerase uses RNA as template Rationale for this paper Before this paper was written, researchers observed that The genomes of certain tumor viruses were made of RNA. However, inhibitors of DNA synthesis prevented infection and transformation by these viruses, and production of virions was sensitive to actinomycin D. Moreover, cells that were infected with the viruses produced DNA that hybridized with the viral RNA. These observations lead scientists to hypothesize that replication of these RNA viruses took place through a DNA intermediate....
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This note was uploaded on 06/08/2010 for the course MCDB 134 taught by Professor Samuels during the Spring '10 term at UCSB.

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Week_7_2009_Baltimore_ANSWERS - Each discussion group no...

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