Lecture16bio

Lecture16bio - How does DNA Recombination occur in nature...

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BIOLOGY 1001 Friday, March 23, 2008 Lecture 16 Chapter 13: Biotechnology Using or altering organisms/cells/biological molecules for practical purposes. Goals of Biotechnology: 1.) 2.) 3.) $$$$$ including Improved plants and animals for agriculture. Efficient production of valuable bio-molecules. Golden Rice 120 Million children low on Vitamin A. This can lead to blindness. Rice is a staple food in many parts of the world. Golden rice is genetically modified to produce beta carotene, which can become Vitamin A. India and the Philippines have permits to plant this rice. What is Recombinant DNA? Transfer of genetic material between organisms. Same or different species. Transgenic: organism w/DNA derived from another species.
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Unformatted text preview: How does DNA Recombination occur in nature? • Sexual Reproduction • Bacterial Transformation • Viral transfer of DNA How does DNA Recombination occur in Nature? Recombination in Bacteria Figure 13-1 Viruses May Transfer Genes Figure 13-2 DNA Recombination in Laboratories • Identify gene(s) of interest. • Isolate the gene(s) of interest. • Produce many copies of that gene. • Express it. Restriction enzymes cut DNA at specific places • Bacteria use restriction enzymes to defend against viral infections. Pg. 250 Tools Needed to Make Recombinant DNA in Laboratories • Scissors – Bacterial Restriction Enzyme • Glue – DNA Ligase • Vector o Bacterial Plasmids o Viruses o Yeast Cells o Other Cells...
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This note was uploaded on 06/09/2010 for the course BIOL 1001 taught by Professor Minor during the Spring '08 term at LSU.

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Lecture16bio - How does DNA Recombination occur in nature...

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