ch_4 - Chapter 4 Dynamics Dynamics of Prokaryotic Growth 1...

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Chapter 4 Dynamics of Prokaryotic Growth 1
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Microbial Growth s Defined as an increase in number s Achieved by binary fission s Generation or doubling time s N t =N 0 x 2 n Fig. 04.03 2
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Microorganism Generation Time Beneckea natriegens 9.6 min Escherichia coli 21 min Generation Times Staphylococcus aureus 28 min Mycobacterium tuberculosis 12 hr Saccharomyces cerevisiae (fungus) 2 hr 3
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Put Into Practice E. coli has a doubling time of about 20 minutes. A cook who forgets to wash his hands after going to the bathroom cuts up a tomato for your pasta dinner. The waiter is The waiter is extremely busy and brings the platter 1 hour after it was initially prepared. The salad contained 200 E. coli cells when it was made. How many are there now? 4
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Obtaining a pure culture s Taking samples from nature and isolating one species Biofilms s s Established by planktonic cells and a slime layer s Pure culture: descended from one cell s Sterile instruments, aseptic technique 5
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Pure cultures s Using streak-plate method, spread culture to single cells s Culture medium: nutrients in liquid nutrients in liquid or solid form s Single cell grows into a colony on solid s Growth on agar plate s Polysaccharide from marine algae s Maintain culture s Restreak cultures regularly s Store long term at -70˚C 6
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This note was uploaded on 06/11/2010 for the course MIBO 2500 taught by Professor Walker during the Spring '08 term at University of Georgia Athens.

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ch_4 - Chapter 4 Dynamics Dynamics of Prokaryotic Growth 1...

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